Fig. 3

Expression of Klf6-SV isoforms and cell proliferation assay in the target clones. a Expression assay of Klf6-SV1 and -SV2. The SE-del clone cells were transfected with pSuper-siSV1, 2 or control pSuper-siLuc. b Cell proliferation assay. [3H] thymidine incorporation was determined at 72 h. Each experiment was performed in triplicate, **p < 0.01. c Expression assay of Klf6-SV isoforms: The SE-non-del clone cells were transfected with pSuper-siSV1,2,3 or control pSuper-siLuc. The SE-non-del clone cells were transfected with pSuper-si-wtKlf6 or control pSuper-siLuc. The results represent the mean of three amplifications, **p < 0.01,*p < 0.05. d Cell proliferation assay. Each experiment was performed in triplicate, **p < 0.01,*p < 0.05,^#p > 0.05