TY - JOUR AU - Abdel Rahman, Mona Mohammed I. PY - 2020 DA - 2020/06/01 TI - Morphological and molecular characterization of Setaria equina in donkeys JO - Beni-Suef University Journal of Basic and Applied Sciences SP - 17 VL - 9 IS - 1 AB - Adult worms of Setaria equina mainly found in the peritoneal cavity of equine. They were nonpathogenic but might induce varied degrees of peritonitis and might migrate to the eye, brain, lung, and scrotum causing lacrimation, blindness, paraplegia, locomotor, and neurological disturbances. Identification by light microscopy is insufficient to differentiate Setaria species, and so scanning electron microscopy (SEM) is required to observe their ultrastructures. The study was performed on 80 donkeys from May 2018 to January 2019 for the detection of microfilaria in blood and the adult worms in the peritoneal cavity. The blood samples were either stained with Giemsa stain or examined by modified Knottā€™s technique for the detection of microfilariae. Adult worms were morphologically characterized based on light microscope and scanning electron microscopy (SEM). PCR was performed targeting the 12S rRNA gene followed by sequencing and phylogenetic analysis. SN - 2314-8543 UR - https://doi.org/10.1186/s43088-020-00046-y DO - 10.1186/s43088-020-00046-y ID - Abdel Rahman2020 ER -