From: Morphological and molecular characterization of Setaria equina in donkeys
Primer | Sequence 5"–3" | Start | Stop | Product length | 1st De. | Amplification | F. Ex. | Reference | ||
---|---|---|---|---|---|---|---|---|---|---|
2nd De. | An. | Ex. | ||||||||
12SF 12SR | 5′-GTT CCA GAA TAA TCG GCT A-3′ | 7484 | 7502 | 408 bp | 94 °C 5 min | 94 °C 1 min | 50 °C 1 min | 72 °C 1 min | 72 °C 10 min | [20] |
5′-ATT GAC GGA TG(AG) TTT GTA CC-3 | 7994 | 7975 | 40 cycles |