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Table 1 PCR cycling program and primers sequences

From: Morphological and molecular characterization of Setaria equina in donkeys

Primer

Sequence 5"–3"

Start

Stop

Product length

1st De.

Amplification

F. Ex.

Reference

2nd De.

An.

Ex.

12SF

12SR

5′-GTT CCA GAA TAA TCG GCT A-3′

7484

7502

408 bp

94 °C 5 min

94 °C 1 min

50 °C 1 min

72 °C 1 min

72 °C 10 min

[20]

5′-ATT GAC GGA TG(AG) TTT GTA CC-3

7994

7975

40 cycles

  1. An. annealing, De. denaturation, F. Ex. final extension