TY - JOUR AU - Olaniyi, Moshood O. AU - Adebiyi, Adeniyi A. AU - Ajayi, Olusola L. AU - Alaka, Olugbenga O. AU - Akpavie, Stephen O. PY - 2020 DA - 2020/05/06 TI - Localization and immunohistochemical detection of swine influenza A virus subtype H1N1 antigen in formalin-fixed, paraffin-embedded lung tissues from naturally infected pigs JO - Beni-Suef University Journal of Basic and Applied Sciences SP - 16 VL - 9 IS - 1 AB - Swine influenza A viruses (SIV) infection is among the leading causes of respiratory diseases in a number of animal species and human, and has been reported to cause substantial losses to pig industry. Previous reports of serological, molecular, and surveillance studies in commercial piggeries in Nigeria indicated the presence of SIV subtypes H1N1 and H3N2 in infected pigs; hitherto, there exists lack of studies on the pulmonary pathology and pathogenicity of SIV in Nigeria. This study investigates the presence of SIV subtype H1N1 antigen in the formalin-fixed paraffin-embedded lung sections obtained from apparently healthy pigs slaughtered at abattoirs located in Lagos, Ogun, and Oyo States, Southwest Nigeria using a streptavidin-biotin (ABC) immunoperoxidase (IP) staining. Two hundred four lungs consisting of 144 grossly pneumonic lungs and 60 apparently normal lungs were randomly collected, fixed in 10% neutral-buffered formalin, embedded in paraffin wax, and processed for histopathological examination and immunohistochemistry. SN - 2314-8543 UR - https://doi.org/10.1186/s43088-020-0039-3 DO - 10.1186/s43088-020-0039-3 ID - Olaniyi2020 ER -