Table 1 Primers sequence for RT-PCR
From: Evaluation of the cytotoxic anticancer effect of polysaccharide of Nepeta septemcrenata
Target gene | Primer sequence: 5′–3′ |
|---|---|
P53 | F: GCAACGGAACACTCTCCATATTTT. R: CAGAGAAGAAACGGTAGCAGAAGA |
P16 | F: CATTCGCTAAGTGCTCGGAGT. R: CTCCTCTTTCTTCCTCCGGTG |
Cyclin -D1 | F: GGAAAGCTTCATTCTCCTTGTTG. R: TCTAGGTAAACCTCTGAGGTCC |
TERT | F: TACGTCGTGGGAGCCAGAAC. R: CCTTCACCCTCGAGGTGAGA |
Fas | F: TCTTTCACTTCGGAGGATTGCT. R:ACTTTCTGTTCTGCTGTGTCTT |
Fas-L | F: GGTCCATGCCTCTGGAATGG. R:CACATCTGCCCAGTAGTGCA |
BCL2 | F: ATGGCGCACGCTGGGAGAAC. R: GCGGTAGCGGCGGGAGAAGT |
Bax | F: TTCATCCAGGATCGAGCAGG. R: CTCTGCAGCTCCATGTTACT |
Caspase-9 | F: TACAGCTGTTCAGACTCTAGTA. R: AAATATGTCCTGGGGTAT |
Caspase-3 | F: TGACAGCCAGTGAGACTTGG. R: GACTCTAGACGGCATCCAGC |
TNF-α | F: ATCCGGGACGTGGAGCTG. R:AAAGTAGACCTGCCCAGAC |
TGF-β | F: AACACATCAGAGCTCCGAGAA. R: GTCAATGTACAGCTGCCGCAC |
VEGF | F: CGGGAACCAGATCTCTCACC. R: AAAATGGCGAATCCAATTCC |
βΒ-actin | F: GGC GGCACCACCATGTACCCT. R: AGG GGCCGGACTCGTCATACT |