Table 1 Sequences and names of forward and reverse primers of five key genes related to glucosinolates and kaempferol synthetic pathway as well as primers used for Eruca sativa CHS PCR amplification and sequencing

MAM1 (E. sativa)

MAM1-F: 5′-ACTCCACCGCAGAAGATTG-3′

MAM1-R: 5′-CCTCATCCACCTCGTTTCC-3

[49]

SUR1 (E. sativa)

SUR1F: 5′-CATTGTTGTGATTAATCCCAAC-3′

SUR1R: 5′-TCCGAATATAGTCTGATCATAA-3

[49]

MYB34 (E. sativa)

MYB34-F: 5′-CTACATGGTGAAGGTGGATGG-3′

MYB34-R: 5′-ATCGGGTCTTAGATAGTTAGCC-3

[49]

MYB51 (E. sativa)

MYB51-F: 5′-CCTTCACACCAACCTCATCAG-3′

MYB51-R: 5′-AACACAAGACTCTCCGAACATC-3

[49]

CHS (Arabidopsis thaliana)

CHS-F: 5′-AAGCTCTCACTCTCCGGT-3'

CHS-R: 5'- TCGTGTGAGTCCCTTGCT-3'

[61] (used for qRT-PCR analysis)

β-actin

Actin-F: 5′-CGCCGCTTAACCCTAAGGCTAACAG-3′

Actin-R: 5′-TTCTCTTTAATGTCACGGACGATTT-3′

[9]

CHS (Arabidopsis thaliana)

CHS-F1: 5′-ACTTCCGCATCACCAACAGT-3′

CHS-R1: 5′-AGAGGGACCACGGAAGGTAA-3′

CHS-F2:5′-TTACCTTCCGTGGTCCCTCT-3′

CHS-R2: 5′-TGTTCTTGGAGATGAGGCCG-3′

This study (used for PCR amplification and sequencing)