Table 2 Primers and probes sequences used in RT-PCR [59]
From: Evolution of RNA viruses from SARS to SARS-CoV-2 and diagnostic techniques for COVID-19: a review
Assay | Gene sequence |
|---|---|
ORF1a (413 bp) | 5′-TTCGGATGCTCGAACTGCACC-3′ (Sense) |
5′-CTTTACCAGCACGTGCTAGAAGG-3′ (Antisense) | |
ORF1b (132 bp) | 5′-TGGGGYTTTACRGGTAACCT-3′ (Forward, Y = C/T; R = A/G) |
5′-AACRCGCTTAACAAAGCACTC-3′ (Reverse, R = A/G) | |
5′-TAGTTGTGATGCWATCATGACTAG-3′ (W = A/T; 5′-FAM/ZEN/3′-IBHQ) | |
N gene (110 bp) | 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) |
5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) | |
5′-GCAAATTGTGCAATTTGCGG-3′ (5′-FAM/ZEN/3′-IBHQ) |