Table 1 Physiochemical parameters of Y-domain

1

Number of amino acids

218

2

Molecular weight

24,632.23

3

Theoretical pI

9.13

4

Total number of negatively charged residues

(Asp + Glu)

18

5

Total number of positively charged residues

(Arg + Lys)

24

6

Formula

C₁₁₀₉H₁₇₁₆N₃₀₆O₃₁₃S₉

7

Total number of atoms

3453

8

Extinction coefficient (assuming all Cys pairs residues form cystines)

40,130

9

Extinction coefficient (assuming all Cys pairs residues are reduced)

39,880

10

Estimated half-life

1 h (mammalian reticulocytes, in vitro) 2 min (yeast, in vivo) 2 min (Escherichia coli, in vivo)

11

Instability index

41.57

12

Aliphatic index

82.75

13

Grand average of hydropathicity (GRAVY)

−0.141