Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Beni-Suef University Journal of Basic and Applied Sciences

Table 1 Primers used in this study to detect the PAHs biodegrading genes

Genes	Size (bp)	Primers		References
acin GS	200	F	ATCCAACCACGTTCAAGGTC	[42]
acin GS	200	R	GCGATCAACAATTTTCGTCA	[42]
tnpA	500–600	F	GCCATTGAAGGTGTCATCCG	[41]
tnpA	500–600	R	AGGTATTCCACGCCATCACG	[41]
tadQ	400	F	ACGATGGTGCTGTTCCGCAA	[41]
tadQ	400	R	TATGAGGCAGGATGGTGACG	[41]
tadQ2	400	F	AGTGCTAAGCTTTTCGTACCGTCAC	[43]
tadQ2	400	R	GGATCCATCGCGTCTCCTAGGTTA	[43]
tadR	400–500	F	GATGGATCCGAGGCGCTGCTC	[43]
tadR	400–500	R	GCTCTAGACGATGCGCACCTTG	[43]
GS2	400	F	GGCTGGATGCTGGCAGATCT	[44]
GS2	400	R	AAGGTGGTTTCCATCTGGCT	[44]
ps pass 2.3	200	F	TGGGTTTCAAGGTTGTGGAT	This study
ps pass 2.3	200	R	GACGTCATTCAAACCCCACT	This study