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Table 4 Comparative between ELISA, PCR, and LAMP for detection of T. gondii in milk and blood samples from lactating female of camels, sheep and goat

From: Rapid diagnosis of Toxoplasma gondii using loop-mediated isothermal amplification assay in camels and small ruminants

Species Types No ELISA PCR LAMP
Sera Milk Blood Milk Blood Milk
 + Ve %  + ve %  + ve %  + Ve %  + ve %  + ve %
Camels LF 41 15/41 (36.58) 12/41 (29.26) 6/41 (14.63) 2/41 (4.8) 6/41 (14.63) 2/41 (4.8)
Sheep LF 117 42/117 (35.89) 40/117 (34.18) 9/117 (7.69) 8/117 (6.83) 9/117 (7.69) 8/117 (6.83)
Goat LF 77 28/77 (36.36) 26/77 (33.7) 7/77 (9.09) 6/77 (7.79) 7/77 (9.09) 6/77 (7.79)
Total number of LF from different animals   235 85/235 (36.17) 78/235 (30.12) 22/235 (9.36) 16/235 (6.8) 22/235 (9.36) 16/235 (6.8)
  1. LF: lactating female; Non LF: Non lactating female (dry or young female); M: Male
  2. This table shows clearly that the prevalence values of T. gondii in sheep and goat milk samples were 26% and 40%, respectively. However, the T. gondii antibody was detected in 12/41 (29.26%) of examined camel milk samples. As seen in Table 4, the seropositive samples were subjected to further examination using PCR and LAMP. The DNA of T. gondii was detected in milk samples of 2/41 (4.8%) camels, 8/117 (6.83%) sheep, and 6/77 (7.79%) goats