Fig. 1
From: Gel express: a novel frugal method quantifies gene relative expression in conventional RT-PCR
Monitoring amplification steps of conventional PCR using gel express method and estimating integrated cycle (IntCyc) value for OsCYP94C2a, OsLOX8, and the reference gene actin. a plotting IntDen values of each PCR amplicon band against corresponding number of cycles. Integrated cycle (IntCyc) was assumed to be slightly beyond the middle of the exponential phase which is estimated to be 29 cycles. b Visualizing PCR amplicon bands for each studied gene where 5 ul aliquots were taken at 18, 20, 22, 24, 26, 28, 30, 32, and 34 cycles. c Amplification plot of the studied gene as directly viewed by QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific, USA). Means represented in a were from at least 2 independent technical replicates presented as Run#1 and Run#2 in (b)