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Fig. 2 | Beni-Suef University Journal of Basic and Applied Sciences

Fig. 2

From: Gel express: a novel frugal method quantifies gene relative expression in conventional RT-PCR

Fig. 2

PCR amplification efficiency determination using gel express and real-time PCR methods. a Integrated densities (IntDen) values were plotted against the log2 values of initial cDNA quantities for the target gene OsCYP94c2a and reference gene actin after 25 cycles of amplification. b Integrated densities (IntDen) values were plotted against the log2 values of initial cDNA quantities for the target gene OsLOX8 and its reference gene actin after 33 cycles of amplification. c crossing points (CP) values were plotted against log2 values of initial cDNA quantities for target genes OsCYP94C2a and OsLOX8, and the reference gene actin. For a, b, and c, slopes were determined then used to calculate PCR efficiency E values according to the formula E = 2[−1/slope] [13]. Means in a, b and c represented at least 3 independent technical replicates. d Representative agarose gel electrophoresis (image with inverted color) used to plot (a) and (b)

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