Fig. 5
From: Gel express: a novel frugal method quantifies gene relative expression in conventional RT-PCR
Photographed-steps protocol of gel express method for attaining integrated densities (IntDen) values of PCR amplicon bands from agarose gel inverted-color image. The presented example illustrates quantifying OsCYP94C2a expression in response to mechanical wounding stress after 0, 30, 60 and 150 min in rice seedlings. The software used was the freeware ImageJ. a opening image by ImageJ software and changing its type to 8-bite grayscale, b and c illustrating scaling image depending on agarose gel well width (in this study it was 0.5 cm), d selecting the area around the band of interest, preferably as shown in the example, then press “t” to open ORI manager, e after selecting all bands then click “measure” button in ROI manager, and f illustrated saving raw data as CSV file