Table 1 PCR primers used to detect polymorphisms of TGF-β1 (+915G/C) and IL6 (−174 G/C)

IL-6 (−174 G/C)

Forward primer (G)

5′-GCACTTTTCCCCCTAGTTGTGTCTTACG-3′

Forward primer (C)

5′-GACGACCTAAGCTTTACTTTTCCCCCTAGTTGTGTCTTGAC-3′

Reverse primer

5′-ATAAATCTTTGTTGGAGGGTGAGG-3′

TGF-β1 +915 G/C

Primer G (sense)

5′-GTG CTG ACG CCT GGC CG-3′

Primer C (sense)

5′-GTG CTG ACG CCT GGC CC-3′

Generic primer (antisense)

5′-GGC TCC GGT TCT GCA CTC-3′

Internal control (forward and reverse)

5′-GCC TTC CCA ACC ATT CCC TTA-3′

5′-TCA CGG ATT TCT GTT GTG TTT C-3′