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Beni-Suef University Journal of Basic and Applied Sciences
Fig. 6 | Beni-Suef University Journal of Basic and Applied Sciences

Fig. 6

From: Molecular docking prediction and in vitro studies elucidate anti-inflammatory effect of Garcinia extract against inducible nitric oxide synthase and cyclooxygenase-2 targets

Fig. 6

Effect of different treatments on RAW264.7 murine macrophage cells. Changes of the morphology were observed under microscope and photographs were taken with Zeiss Zen blue inverted microscope. a Control, b LPS treatment, c GME(115 µg/ml) + LPS treatment d GME(230 µg/ml) + LPS treatment e Garcinol(6 µM) + LPS treatment f Garcinol(12 µM) + LPS treatment g HCA(17.5 µg/ml) + LPS treatment h HCA(35 µg/ml) + LPS treatment. RAW 264.7 cells were cultured in 100-mm dishes at a density of 106cells/cm2 and allowed to settle for 16 h. After 16 h of incubation, the medium was discarded and the cells were pre-treated as mentioned above. The cells were then stimulated with 1 µg/ml LPS for 24 h except for the control group. A typical experimental result of three independent experiments is represented

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