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Beni-Suef University Journal of Basic and Applied Sciences
Fig. 4 | Beni-Suef University Journal of Basic and Applied Sciences

Fig. 4

From: Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization

Fig. 4

Three-dimensional (3D) response surface plots showing the effects of variables (temperature, 6-BA and NAA) on puerarin, daidzin, and daidzein synthesis in P. thomsonii callus culture. A, B The model generated by the CCD on production of puerarin (%), C production of daidzin (%), D production of daidzein (%). The optimum temperature ranging from 20.0 to 28.0 °C, 6-BA and NAA concentrations between 1.00 and 2.50 mg/L, respectively. Color-coding indicates high (red) and low (green) responses

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