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Beni-Suef University Journal of Basic and Applied Sciences

Table 1 Calibration curve analysis for puerarin, daidzin and daidzein

From: Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization

Analyte

Calibration curve

R2

Linear range (mg/mL)

LOD (mg/mL)

LOQ (mg/mL)

Puerarin

y = 24.38686x + 214.09593

0.99994

1.0–36.0

0.15

0.28

Daidzin

y = 29.93579x + 130.22225

0.99980

5.0–72.0

0.52

1.50

Daidzein

y = 12.3412x + 13.02450

0.99941

1.0–15.0

0.35

0.50

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