Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization

Li, Yu; Saravana Kumar, Pachaiyappan; Liu, Yu; Qiu, Jiao; Ran, Yalan; Yuan, Mingyuan; Fang, Xinyue; Tan, Xuhui; Zhao, Renjun; zhu, Ji; He, Meijun

doi:10.1186/s43088-022-00220-4

Beni-Suef University Journal of Basic and Applied Sciences

Table 2 Central composite design matrix of three variables (temperature, 6-BA and NAA) in coded and actual concentration units along with the study of responses with respect to puerarin, daidzin and daidzein synthesis in P. thomsonii callus culture

From: Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization

Std	Run	Independent variables			Response values
Std	Run	Tem (°C)	6-BA (mg/L)	NAA (mg/L)	Puerarin (%)	Daidzin (%)	Daidzein (%)
8	1	28.0 (+ 1)	2.5 (+ 1)	2.50 (+ 1)	0.0685 ± 0.0088	0.3063 ± 0.0254	0.0430 ± 0.0045
9	2	17.3 (− 1.68)	1.75 (0)	1.75 (0)	0.0750 ± 0.0094	0.2875 ± 0.0342	0.0250 ± 0.0036
16	3	24.0 (0)	1.75 (0)	1.75 (0)	0.1560 ± 0.0143	0.3909 ± 0.0458	0.0620 ± 0.0065
1	4	20.0 (− 1)	1.00 (− 1)	1.00 (− 1)	0.0315 ± 0.0063	0.1711 ± 0.0123	0.0180 ± 0.0078
17	5	24.0 (0)	1.75 (0)	1.75 (0)	0.1354 ± 0.0153	0.4511 ± 0.0458	0.0690 ± 0.0094
15	6	24.0 (0)	1.75 (0)	1.75 (0)	0.1654 ± 0.0241	0.5206 ± 0.0463	0.0510 ± 0.0057
3	7	20.0 (− 1)	2.50 (+ 1)	1.00 (− 1)	0.0612 ± 0.0073	0.1889 ± 0.0243	0.0090 ± 0.0087
4	8	28.0 (+ 1)	2.50 (+ 1)	1.00 (− 1)	0.0356 ± 0.0026	0.2545 ± 0.0378	0.0080 ± 0.0049
7	9	20.0 (− 1)	2.50 (+ 1)	2.50 (+ 1)	0.0557 ± 0.0037	0.1145 ± 0.0145	0.0420 ± 0.0037
11	10	24.0 (0)	0.49 (− 1.68)	1.75 (0)	0.0645 ± 0.0095	0.1856 ± 0.0183	0.0450 ± 0.0098
10	11	30.7 (+ 1.68)	1.75 (0)	1.75 (0)	0.0167 ± 0.0042	0.3689 ± 0.0253	0.0310 ± 0.0082
6	12	28.0 (+ 1)	1.00 (− 1)	2.50 (+ 1)	0.1508 ± 0.0115	0.3974 ± 0.0328	0.0330 ± 0.0048
20	13	24.0 (0)	1.75 (0)	1.75 (0)	0.1600 ± 0.0187	0.4207 ± 0.0661	0.0530 ± 0.0018
5	14	20.0 (− 1)	1.00 (− 1)	2.50 (+ 1)	0.0655 ± 0.0068	0.3113 ± 0.0502	0.0110 ± 0.0034
14	15	24.0 (0)	1.75 (0)	3.01 (+ 1.68)	0.1045 ± 0.0121	0.2613 ± 0.0467	0.0310 ± 0.0048
12	16	24.0 (0)	3.01 (+ 1.68)	1.75 (0)	0.0511 ± 0.0069	0.2635 ± 0.0183	0.0410 ± 0.0079
19	17	24.0 (0)	1.75 (0)	1.75 (0)	0.1490 ± 0.0211	0.4807 ± 0.0834	0.0480 ± 0.0041
13	18	24.0 (0)	1.75 (0)	0.49 (− 1.68)	0.0327 ± 0.0071	0.1667 ± 0.0282	0.0230 ± 0.0028
2	19	28.0 (+ 1)	1.00 (− 1)	1.00 (− 1)	0.0272 ± 0.0054	0.2167 ± 0.0516	0.0340 ± 0.0019
18	20	24.0 (0)	1.75 (0)	1.75 (0)	0.1601 ± 0.0214	0.4414 ± 0.0918	0.0540 ± 0.0083

Data are expressed as means ± standard deviations (SD) of three independent assays

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