Table 4 Analysis of variance (ANOVA) of the influence of temperature, 6-BA and NAA concentration on puerarin, daidzin and daidzein synthesis
Source of variation | Puerarin (%) | Daidzin (%) | Daidzein (%) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sum of squares | df | Mean square | F value | p value | Sum of squares | df | Mean square | F value | p value | Sum of squares | df | Mean square | F value | p value | |
Model | 0.044 | 6 | 7.291 × 10−3 | 52.620 | < 0.0001* | 0.240 | 6 | 0.039 | 17.840 | < 0.0001* | 4.817 × 10−3 | 5 | 9.634 × 10−4 | 14.510 | < 0.0001* |
Residuals | 1.801 × 10−3 | 13 | 1.386 × 10−4 | – | – | 0.029 | 13 | 2.212 × 10−3 | – | – | 9.298 × 10−4 | 14 | 6.642 × 10−5 | – | – |
Lack of fit | 1.224 × 10−3 | 8 | 1.530 × 10−4 | 1.320 | 0.3944ns | 0.018 | 8 | 2.301 × 10−3 | 1.110 | 0.4750ns | 6.230 × 10−4 | 9 | 6.922 × 10−5 | 1.130 | 0.4722ns |
Pure Error | 5.775 × 10−4 | 5 | 1.155 × 10−4 | – | – | – | 5 | 2.070 × 10−3 | – | – | 3.068 × 10−4 | 5 | 6.137 × 10−5 | – | – |