Fig. 1

a Light micrographs (LM) a transverse section of a control group of rats of testis showing seminiferous tubules lined with basement membrane (BM) had myoid cells (m), interstitial tissue (I) among the tubules, spermatogonia appeared as small, rounded cells (g), primary spermatocyte as large, rounded nuclei (c). Spermatids as small, rounded cells with pale nuclei (t); Sertoli cells have triangular nuclei attached to the basement membrane (r), sperms (S) in the center, and Leydig cells (L). (Hematoxylin and Eosin × 400). b Light micrographs (LM) a transverse section of second groups of rats of testis showing seminiferous tubules lined with basement membrane (BM) had myoid cells (m), interstitial tissue (I) among the tubules, spermatogonia appeared as small, rounded cells (g), primary spermatocyte as large, rounded nuclei (c). Spermatids as small, rounded cells with pale nuclei (t). Sertoli cells had triangular nuclei attached to the basement membrane (r), sperms (S) in the center, and Leydig cells (L). (Hematoxylin and Eosin × 400). c Light micrographs (LM) of a transverse section testis of rat third group showed well-defined disorganization with necrosis, vacuoles (v) of germ cells (g), degenerated Leydig cells (L), and degenerated basement membrane (BM), congested blood vessels (bv). (Hematoxylin and Eosin × 400). d Light micrographs (LM) of the transverse section of testis of the fourth group showed the normal structure of spermatogonia cells (g) with normal basement membrane (BM); Sertoli cells (r) and Leydig cells (L) normal spermatocytes c), spermatid (t) and sperms (S). (Hematoxylin and Eosin × 400)