Chromosomal studies on drug resistance genes in extended spectrum β-lactamases producing-Klebsiella pneumoniae isolated from equine

Fawzy, Nehal M.; Abd Elmawgoud, Shaimaa R. A.; El-Shafii, Soumaya S. A.; Farag, Azza N.; Osman, Kamelia M.

doi:10.1186/s43088-022-00247-7

Beni-Suef University Journal of Basic and Applied Sciences

Table 1 Primers sequences, target genes, amplicon sizes and cycling conditions

From: Chromosomal studies on drug resistance genes in extended spectrum β-lactamases producing-Klebsiella pneumoniae isolated from equine

Target gene	Primers sequences	Amplified segment (bp)	Primary denaturation	Amplification (35 cycles)			Final extension	References
Target gene	Primers sequences	Amplified segment (bp)	Primary denaturation	Secondary denaturation	Annealing	Extension	Final extension	References
KP_16S (NM3)	ATGTCGCAAGACCAGAGTGG	657	94 °C/1 min	94 °C/10 s	58 °C/5 s	60 °C/4 min	60 °C/10 min	[22]
KP_16S (NM3)	GCACAACCTCCAAATCGACA	657	94 °C/1 min	94 °C/10 s	58 °C/5 s	60 °C/4 min	60 °C/10 min	[22]
aadA1	TATCAGAGGTAGTTGGCGTCAT	484	94 °C/5 min	94 °C/30 s	54 °C/40 s	72 °C/45 s	72 °C/10 min	[23]
aadA1	GTTCCATAGCGTTAAGGTTTCATT	484	94 °C/5 min	94 °C/30 s	54 °C/40 s	72 °C/45 s	72 °C/10 min	[23]
acrAB	ATCAGCGGCCGGATTGGTAAA	312	94 °C/5 min	94 °C/30 s	53 °C/40 s	72 °C/40 s	72 °C/10 min	[18]
acrAB	CGGGTTCGGGAAAATAGCGCG	312	94 °C/5 min	94 °C/30 s	53 °C/40 s	72 °C/40 s	72 °C/10 min	[18]
bla_TEM	ATCAGCAATAAACCAGC	516	94 °C/5 min	94 °C/30 s	54 °C/40 s	72 °C/45 s	72 °C/10 min	[24]
bla_TEM	CCCCGAAGAACGTTTTC	516	94 °C/5 min	94 °C/30 s	54 °C/40 s	72 °C/45 s	72 °C/10 min	[24]
bla_CTX	ATG TGC AGY ACC AGT AAR GTK ATG GC	593	94 °C/5 min	94 °C/30 s	54 °C/40 s	72 °C/45 s	72 °C/10 min	[25]
bla_CTX	TGG GTR AAR TAR GTS ACC AGA AYC AGC GG	593	94 °C/5 min	94 °C/30 s	54 °C/40 s	72 °C/45 s	72 °C/10 min	[25]
bla_VIM	AGTGGTGAGTATCCGACA	280	94 °C/5 min	94 °C/30 s	55 °C/30 s	72 °C/30 s	72 °C/7 min	[26]
bla_VIM	ATGAAAGTGCGTGGAGAC	280	94 °C/5 min	94 °C/30 s	55 °C/30 s	72 °C/30 s	72 °C/7 min	[26]
bla_NDM1	GGCGGAATGGCTCATCACGA	287	94 °C/5 min	94 °C/30 s	55 °C/30 s	72 °C/30 s	72 °C/7 min	[26]
bla_NDM1	CGCAACACAGCCTGACTTTC	287	94 °C/5 min	94 °C/30 s	55 °C/30 s	72 °C/30 s	72 °C/7 min	[26]
bla_KPC	ATGTCACTGTATCGCCGTCT	892	94 °C/5 min	94 °C/30 s	55 °C/40 s	72 °C/50 s	72 °C/10 min	[26]
bla_KPC	TTTTCAGAGCCTTACTGCCC	892	94 °C/5 min	94 °C/30 s	55 °C/40 s	72 °C/50 s	72 °C/10 min	[26]
qnrB	ATGACGCCATTACTGTATAA	562	94 °C/5 min	94 °C/30 s	53 °C/40 s	72 °C/45 s	72 °C/10 min	[27]
qnrB	GATCGCAATGTGTGAAGTTT	562	94 °C/5 min	94 °C/30 s	53 °C/40 s	72 °C/45 s	72 °C/10 min	[27]
qnrS	ATGGAAACCTACAATCATAC	491	94 °C/5 min	94 °C/30 s	48 °C/40 s	72 °C/45 s	72 °C/10 min	[28]
qnrS	AAAAACACCTCGACTTAAGT	491	94 °C/5 min	94 °C/30 s	48 °C/40 s	72 °C/45 s	72 °C/10 min	[28]
Omp K35	GCAATATTCTGGCAGTGGTGATC	109	94 °C/5 min	94 °C/30 s	60 °C/30 s	72 °C/30 s	72 °C/7 min	[29]
Omp K35	ACCATTTTTCCATAGAAGTCCAGT	109	94 °C/5 min	94 °C/30 s	60 °C/30 s	72 °C/30 s	72 °C/7 min	[29]

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