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Table 2 Fractional factorial design for optimization of adsorption and results analysis

From: Preparation and characterization of polymyxin B- and histidine-coupled magnetic nanoparticles for purification of biologics from acquired endotoxin contamination

Ligand type Ligand conc. (µg/ml) CBE conc. (EU/ml) pH of incubation Temp of incubation (°C) Time of incubation (min) %Removal
HIS 275 500,000 6.5 25 17.5 71.90
PMB 275 500,000 6.5 25 17.5 51.24
HIS 500 250,000 9 2–8 5 67.68
PMB 275 500,000 6.5 2–8 17.5 24.79
HIS 50 750,000 4 25 5 67.35
HIS 500 750,000 9 25 30 40.30
PMB 500 250,000 4 25 5 56.25
HIS 50 250,000 4 2–8 30 62.50
PMB 50 750,000 9 2–8 5 54.76
HIS 275 500,000 6.5 2–8 17.5 85.12
PMB 500 750,000 4 2–8 30 43.88
PMB 50 250,000 9 25 30 65.85