Chain(s) | Mutation(s) | Average distance (Å) | Individual ΔΔGBinding (Kcal/mol) | Predicted ΔΔGBinding (Kcal/mol) | Effect |
---|---|---|---|---|---|
H | L219A V225A L233A | 9.75 | − 1.39 − 0.22 − 0.43 | − 1.15 | Decreasing affinity |
H | K234A L238A | 6.16 | − 2.15 − 1.09 | − 2.88 | Decreasing affinity |
M | L94A L163E | 4.84 | − 1.61 − 1.19 | − 2.09 | Decreasing affinity |