From: Optimizing aseptic and serum milieu for the isolation of human whole umbilical cord tissue-derived mesenchymal stem cells
Primary culture
Passage
Initial seed
Harvest at P0
Plate at P1
Final cells at P1
Isolated cells from a single cord plated on T75 Flask
1 × 106 cells MSCs
1.27 × 107 MSCs in 2-cellSTACK chamber
2.48 × 108 MSCs
Day 0
Day 9–10
Day 15–16