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Table 1 Primer sequences for amplification of IL-10 SNPs at locations (− 1082 G/A and − 819 C/T)

From: Gene polymorphisms of interleukin 10 (− 819 C/T and − 1082 G/A) in women with ovarian cancer

IL-10 SNP position

Primera

Sequence

PCR product size (bp)

 − 1082G/A

G (sense)

5-CTACTAAGGCTTCTTTGGGAG-3'

258 bp

 

A (sense)

5-ACTACTAAGGCTTCTTTGGGAA-3

 
 

Generic (antisense)

5-CAGTGCCAACTGAGAATTTGG-3

 

 − 819C/T

C (sense)

5-CCCTTGTACAGGTGATGTAAC-3

233 bp

 

T (sense)

5-ACCCTTGTACAGGTGATGTAAT-3

 
 

Generic (antisense)

5-AGGATGTGTTCCAGGCTCCT-3

 

Control

Internal control (sense)

5-GCCTTCCCAACCATTCECTTA-3

429 bp

 

Internal control (antisense)

5-TCACGGATTTCTGTTGTGTTTC-3