Fig. 2

HIF-1 α immunohistochemistry positive control (breast invasive duct carcinoma), ×400 (A). Astrocytoma, IDH1 mutant, WHO grade 2, negative HIF1 α immunohistochemistry, ×400 (B). Astrocytoma, IDH1-mutant, WHO grade 3 with abnormal mitosis, HIF1-α positive nuclear staining (score 2), ×400 (C). Astrocytoma, IDH1-mutant, WHO grade 4, HIF1 α positive, palisading necrosis (score-3) ×200 (D). Glioblastoma NOS, WHO grade 4. HIF1α positive nuclear staining. Cells around blood vessels ×400 (E)