Table 1 Primers sequences used for qRT-PCR analysis
Gene symbol | Accession number | Primer sequence | Product size (bp) |
|---|---|---|---|
Cytochrome P450 17 A1 (CYP17A1) | NM_012753.3 | F: -5′‐ ACT GAG GGT ATC GTG GAT GC ‐3′ | 187 |
R:-5′‐ CCG TCA GGC TGG AGA TAG AC ‐3′ | |||
Cytochrome P450 11 A1 (CYP11A1) | NM_017286.3 | F:- 5′- GCT GGAAGG TGT AGC TCA GG -3′ | 224 |
R:- 5′- CAC TGG TGT GGA ACA TCT GG -3′ | |||
Steroidogenic Acute Regulatory (StAR) protein | NM_031558.3 | F: -5ʹ- GCC TGA GCA AAG CGG TGT C -3ʹ | 180 |
R:-5ʹ- CTG GCG AAC TCT ATC TGG GTCTGT -3ʹ | |||
Aromatase (CYP19A1) | NM_017085.3 | F:- 5ʹ-CTGCTGATCATGGGCCTCCT-3ʹ | 340 |
R:- 5ʹ- CTCCACAGGCTCGGGTTGTT-3ʹ | |||
Glyceraldehyde3-phosphate dehydrogenase(GAPDH) | NM_017008.4 | F:- 5′-ACCACAGTCCATGCCATCAC-3′ | 452 |
R:- 5′-TCCACCACCCTGTTGCTGTA-3′ |