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Table 5 The results of calculating the minimum free energy for predicted miRNAs using Mfold

From: Employing in silico investigations to determine the cross-kingdom approach for Curcuma longa miRNAs and their human targets

miRNAs structure

ΔG (free energy)

ΔH (enthalpy)

ΔS (entropy)

Tm (°C)

miRNA 167

− 2.90 kcal/mol

− 32.40 kcal/mol

− 95.1 cal/K.mol

67.4

miRNA 129

− 7.50 kcal/mol

–

–

–

miRNA 756

− 0.60 kcal/mol

− 26.70 kcal/mol

84.1 cal/K.mol

44.1

miRNA 1525

0.20 kcal/mol

− 15.90 kcal/mol

− 51.9 cal/K.mol

33.1

miRNA 1568

− 3.70 kcal/mol

− 43.00 kcal/mol

− 126.7 cal/K.mol

66.1

  1. Standard errors are roughly ± 5%, ± 10%, ± 11%, and 2–4 °C for free energy, enthalpy, entropy, and Tm, respectively