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Table 2 Effect of VA on the levels of biochemical parameters and Johnsen scores in testicular tissues of control and experimental rats

From: Vanillic acid attenuates testicular ischemia/reperfusion injury in rats

 

Sham control

T/D

T/D + VA (50 mg/kg)

T/D + VA (100 mg/kg)

MDA (nmol/mg protein)

9.65 (7.98–10.4)

29.6 (23.4–31.2)a

11.4 (9.18–20.1)b

9.90 (7.30–12.8)b

TOS (µmol H2O2 equivalent/L)

18.9 (14.2–27.6)

53.9 (50.7–57.9)a

27.6 (17.2–40.1)b

20.2 (15.6–26.7)b,c

TAS (mmol trolox equivalent/L)

1.24 (1.19–1.29)

0.70 (0.67–0.75)a

1.00 (0.89–1.10)a,b

1.17 (1.07–1.19)b

OSI (arbitrary unit)

1.55 (1.11–2.22)

7.52 (7.05–8.33)a

2.62 (1.94–3.78)a,b

1.73 (1.42–2.31)b

8-OHdG (ng/mg protein)

4.87 (3.88–5.64)

14.1 (11.7–15.1)a

8.36 (6.79–11.2)a,b

4.79 (4.23–5.62)b,c

SOD (ng/mg protein)

1.49 (1.40–1.76)

0.54 (0.44–0.62)a

0.83 (0.64–1.12)a,b

1.00 (0.89–1.52)b

CAT (mIU/mg protein)

7.20 (6.25–8.48)

3.10 (2.63–3.30)a

3.75 (2.78–5.20)a

7.15 (5.85–8.53)b,c

HMGB1 (pg/mg protein)

46.0 (39.8–49.3)

207.1 (175.0–226.6)a

82.5 (47.1–129.9)a,b

56.1 (50.6–58.5)b

NF-κB p65 (pg/mg protein)

105.5 (96.5–114.8)

307.9 (285.1–323.9)a

194.7 (145.4–314.3)a

118.8 (106.9–139.9)b,c

TNF-α (pg/mg protein)

0.14 (0.12–0.15)

0.74 (0.66–0.98)a

0.38 (0.21–0.91)a

0.10 (0.04–0.26)b,c

IL-6 (pg/mg protein)

30.6 (26.2–35.9)

165.3 (156.0–189.6)a

91.1 (80.1–109.2)a,b

31.9 (28.5–38.3)b,c

MPO (ng/mg protein)

0.74 (0.65–0.80)

1.76 (1.58–1.93)a

1.03 (0.60–1.54)b

0.73 (0.54–0.80)b

GRP78 (pg/mg protein)

34.5 (27.6–37.9)

197.6 (163.5–223.4)a

120.2 (85.2–159.0)a,b

24.1 (21.0–36.5)b,c

ATF6 (pg/mg protein)

47.6 (33.4–58.7)

174.1 (162.8–181.7)a

81.7 (60.1–120.6)a,b

45.6 (38.5–51.8)b,c

CHOP (pg/mg protein)

123.4 (106.4–146.1)

323.2 (257.2–343.8)a

187.4 (136.1–281.0)a,b

145.5 (119.7–157.8)b

Caspase-3 (ng/mg protein)

0.16 (0.09–0.26)

0.99 (0.74–1.08)a

0.67 (0.47–0.86)a

0.26 (0.18–0.28)b,c

Johnsen scores

9.5 (8–10)

7 (6–8)a

7.5 (7–8)a

8 (8–9)b,c

  1. T/D, torsion/detorsion; VA, vanillic acid; MDA, malondialdehyde; TOS, total oxidant status; TAS, total antioxidant status; OSI, oxidative stress index; 8-OHdG, 8-hydroxy-2′-deoxyguanosine; SOD, superoxide dismutase; CAT, catalase; HMGB1, high-mobility group box 1; NF-κB, nuclear factor kappa B; TNF-α, tumor necrosis factor-alpha; IL-6, interleukin-6; MPO, myeloperoxidase; GRP78, 78-kDa glucose-regulated protein; ATF6, activating transcription factor 6; CHOP, C/EBP homologous protein
  2. P values according to Kruskal–Wallis variance analysis and followed by the Mann–Whitney U test
  3. Data were expressed as median as medians with a 25th and 75th percentile interquartile range (IQR)
  4. ap < 0.05 compared with control group
  5. bp < 0.05 compared with T/D group
  6. cp < 0.05 compared with T/D + VA (50 mg/kg) group