Fig. 2

A general configuration of the LFIA test strip. To initiate LFIA, the sample of interest is applied to the sample pad and then flows through the sample pad to the conjugate release pad, rehydrating the analyte-specific antibodies or the attached virus antigen. The associated antibody or antigen, together with its target, flows towards the detector zone of the strip after the contacts. As the complex moves up the membrane, it passes the immobilised analyte-specific antibody, which recognises and binds to it, resulting in a coloured line. The control antibody recognises and captures the control well portion of the antibody detector, resulting in a coloured line that eventually serves as a control. Results are interpreted on the reaction matrix as the presence or absence of lines of captured conjugate, read either by Ching [56]