Table 1 Assay parameters for the determination of AZN and RSGL by the proposed methods

λ_nm

238

λem 528

λem 528

λem 528

λem 528

Conc. range (μg/mL)

5–30

0.001–0.005

0.001–0.005

0.001–0.005

0.001–0.005

Regression equation

 a^a

-0.0364

15.298

1.041

31.59

0.755

 b^b

0.012

5178

16.68

81.95

4.805

 r^c

0.9996

0.9995

0.9998

0.9997

0.9997

 S_a

0.00338

0.302

0.4507

0.654

0.036

 S_b

0.00017

91.14

0.00149

1.979

0.108

 S_b²

2.89 × 10⁻⁸

8306.49

2.22 × 10⁻⁶

3.916

0.0118

 S_b %

1.491

0.0058

0.0089

0.798

0.749

 LOD

0.928

0.00019

0.0002

0.026

0.0247

 LOQ

2.81

0.00058

0.0008

0.079

0.0749

 Significance F

0.00022235

1.2

4.33

0.0153

0.0144

 F

4495.82

3227.21

1369.82

1714.1

1948.43

 Accuracy (mean ± RSD%)

99.60 ± 0.82

100.29 ± 0.18

99.72 ± 0.33

99.84 ± 0.42

101.03 ± 0.44

Precision (RSD%)

 Intraday precision

1.07

0.17

0.40

0.33

0.49

 Inter day precision

0.58

0.19

0.41

0.51

0.36