Fig. 3
From: The anti-tumor drug 2-hydroxyoleic acid regulates the oncogenic potassium channel Kv10.1
2OHOA and astemizole synergistically decreases cell proliferation of HEK-293 cells expressing the Kv10.1 channel. A–C the effect of 200 µM 2OHOA on cell proliferation was determined using the MTT assay at 24 (A), 48 (B) and 72 h (C) on HEK-293 cells expressing the Kv10.1 channel (n = 6). D Effect of 200 µM 2OHOA on proliferation of HEK-293 cells expressing the non-conducting mutant Kv10.1-F456A (n = 6). E Effect of 200 µM 2OHOA on proliferation of HEK-293 cells not expressing Kv10.1 channels (n = 5). Asterisks indicate significant effects compared with controls (*p < 0.05), # indicate a significant effect of the combination 2OHOA + Astemizole compared with both drugs applied alone (#p < 0.05), NS: Not significant. Data are represented as mean ± SEM