Table 5 ISSR profile analysis using 10 primers in Allium cepa treated with tap water as control, 25%, 50% and 100% of mixed wastewater at 12th day after germination
No. | ISSR primers name | Primers sequence (5′-3′) | Total bands | Range of size (bp) | Monomorphic bands | Polymorphic bands | % Polymorphism |
|---|---|---|---|---|---|---|---|
1 | UBC 808 | AGAGAGAGAGAGAGAGC | 10 | 210–974 | 4 | 6 | 60 |
2 | UBC 809 | AGA GAG AGA GAG AGAGG | 7 | 210–914 | 3 | 4 | 57.14 |
3 | UBC 810 | GAGAGAGAGAGAGAGAT | 10 | 218–994 | 4 | 6 | 60 |
4 | UBC 818 | CACACACACACACACAG | 8 | 218–1000 | 5 | 3 | 37.5 |
5 | UBC 824 | TCTCTCTCTCTCTCTCTCG | 12 | 218–993 | 5 | 7 | 58.33 |
6 | UBC 826 | ACACACACACACACACC | 13 | 121–973 | 7 | 6 | 46.54 |
7 | UBC 836 | AGAGAGAGAGAGAGAGYA | 15 | 78–995 | 8 | 7 | 46.67 |
8 | UBC 857 | ACACACACACACACACYG | 11 | 107–1008 | 7 | 4 | 36.36 |
9 | UBC 884 | HBH AGA GAG AGA GAG AG | 11 | 99–1008 | 7 | 4 | 36.36 |
10 | UBC 886 | VDV (CT)7 | 11 | 94–977 | 4 | 7 | 63.64 |
Total | Â | 108 | Â | 54 | 54 | 50.25% | |