Biotechnological potentials of halophilic microorganisms and their impact on mankind

Background Halophiles are extremophilic organisms represented by archaea, bacteria and eukaryotes that thrive in hypersaline environment. They apply different osmoadaptation strategies to survive in hostile conditions. Habitat diversity of halophilic microorganisms in hypersaline system provides information pertaining the evolution of life on Earth. Main body The microbiome-gut-brain axis interaction contributes greatly to the neurodegenerative diseases. Gut resident halophilic bacteria are used as alternative medication for chronic brain diseases. Halophiles can be used in pharmaceuticals, drug delivery, agriculture, saline waste water treatment, biodegradable plastic production, metal recovery, biofuel energy generation, concrete crack repair and other sectors. Furthermore, versatile biomolecules, mainly enzymes characterized by broad range of pH and thermostability, are suitable candidate for industrial purposes. Reflectance pattern of halophilic archaeal pigment rhodopsin is considered as potential biosignature for Earth-like planets. Short conclusions This review represents important osmoadaptation strategies acquired by halophilic archaea and bacteria and their potential biotechnological applications to resolve present day challenges. Graphical Abstract


Background
Microorganisms inhabiting different types of extreme environmental niches have been termed as thermophiles, psychrophiles, piezophiles, xerophilies, acidophile, alkaliphiles and halophiles. This successful occupancy of life form in such adverse environment have drawn the attention to the scientific community for potential applications of their bioactive molecules to be used in medicine, agriculture, bioenergy and other industries. Among them, halophilic microorganisms are characterized by the presence of major life forms in saline environment. Halophiles require a minimum of 1 M salt for growth and can proliferate in diverse range of salt concentrations. Depending on optimal salt requirement for growth they are classified as-slight (0.34-0.85 M NaCl), moderate (0.85-3.4 M NaCl) and extreme halophiles (3.4-5.1 M NaCl) [1]. A halotolerant microorganism has no absolute requirement of salt, is able to grow in the presence or absence of high salt concentration. Extreme halotolerant microorganisms are considered to grow above 2.5 M salt.
Halophiles that are mainly thrive under broad spectrum of extreme environments like-pH, salinity, temperature are considered as poly-extremophilic microorganisms. Haloalkaliphilic bacteria such as Desulfonatronospira thiodismutans, Deltaproteobacteria sp. are adapted to grow under high salt and alkaline pH [2].
With the help of advanced metabolic engineering tools, efficient microbial cell factory has been developed. Expression of the ice-nucleation gene (inaZ) from Pseudomonas syringae established in Halomonas strains resulting recombinant proteins that have great potential as alternative cell factories [5].
Study of halophilic and halotolerant prokaryotes (archaea and bacteria) is often neglected and seems to be less promising than eukaryotic alga Dunaliella salina or fungus Wallemia ichthyophaga. The halophilic properties are widespread in all three domains of life i.e., bacteria, archaea, and eukarya. Although cellular life in hypersaline system is represented largely by prokaryotes, eukaryotic organisms are also common. Halophiles can easily be isolated from subterranean solar brine, Rajasthan, India [6]; salt marshes, saline soil saltern crystallizer ponds in Eilat, Israel; hypersaline soda lake, Magadi in Kenya, Africa; brine pool in the Sinai Peninsula, Egypt [7]; microbial mat in a pond of Mediterranean coast; Salinde-Giraud in France [8] and Great Salt Lake or Dead Sea in Utah, USA. In a diverge scenario, cold saline lakes in the Vestfold Hills region of ice-free areas in east Antarctica have contributed resourceful insights of hypersaline life [9].
Osmoregulatory mechanism of halophilic microbes to proliferate constantly in acute salt concentration by stabilizing osmolytes or by ion balance with external environment, brought halophiles into a new arena of biotechnological applications. Considering the fact of desiccation tolerance, and survival for long span of time, it is now thoroughly studied in the discipline of astrobiology, assuming their similar existence in Mars. Adaptation for ionic cytoplasm of specially modified acidic ribosomes and stabilizing protein machinery are making them exciting choice for industrial enzyme processing. Bioactive substances are of countless pharmacological interest and therapeutic values. Halophilic lactic acid bacteria (LAB), Tetragenococcus halophilus has been reported from soy sauce moromi in Japan. This LAB possesses an immunomodulatory activity that exerts T-helper type -1 (Th1) immunity in humans. Thus, development of probiotics with this strain seems favourable for controlling allergic rhinitis in human [10]. Glycolipids compounds exhibiting antiviral activity against enveloped viruses, isolated from halotolerant Rhodococcus sp., can be a potential candidate in the treatment of herpes simplex virus (HSV-1) and human corona viruses (SARS-CoV-2) [11]. Along with this, haloarchaea, Natrialba sp. produce C50 carotenoid bacterioruberin, which showed robust activity against hepatitis C virus (HCV) and hepatitis B virus (HBV) [12]. This unique property could be used to formulate drugs for cancer and viral hepatitis. This review employed survival strategies of halophilic bacteria and archaea to withstand extreme salt stresses and the biomolecules produced are of great interest for biotechnological purposes. Overall, the review work generated evidences to discover basic life principles hidden under salt both in this planet earth and beyond where they can live reasonably.

Long-term adaptation to saline environments
Hypersaline environment has remained a reservoir of life since the primordial days of life on earth. In those early days, salinity was one of the first chemical stresses encountered by early life and therefore, halophiles have evolved some strategies to maintain protoplasmic viability and ion balance. The mechanism used by salt-tolerant microorganisms to withstand the high saline environment are diverse as well. Some general phenomena are as follows-(i) Halophilic bacteria like, Halobacillus halophilus, have adapted mainly two mechanisms underlying salt stress-"salt in/out cytoplasm" to maintain osmotic balance and accumulation of compatible solutes or "organic-osmolytes" such as glycine betaine, ectoine and hydroxyectoine, which are depicted in Fig. 1.
Hypo-osmotic shock protection by aquaporins and cryoprotectants has also been served along with these two primary mechanisms. (ii) Intracellular sodium and potassium ion concentrations are regulated by light driven inward directed chloride pump halorhodopsin in Halobacterium salinarium and Natronomona spharaonic [13] (iii) The halotolerant unicellular green algae Dunaliella salina can tolerate high salinities (0.5-5 M NaCl) by forming large amount of organic solute, glycerol in a salt-dependent manner [14]. Dunaliella sp. maintain low salt concentrations in their cytoplasm as well as in their chloroplast. Glycerol increases osmotic pressure of the cytoplasm by increasing the accumulation of other cytoplasmic solutes, thus preventing fluid loss [14]. (iv) Reduction in hydrophobic amino acids frequencies and greater propensity to form random coiled structure over α helices, is undoubtably determining factor of saline adaptation [15]. (v) Halophilic fungi, Wallemia ichthyophaga maintain low intracellular Na + concentrations by accumulating glycerol as main compatible solute. Smaller amounts of arabitol and mannitol were also reported [16].

Biotechnological importance of halophilic archaea and bacteria for mankind
This study appraises the application of halophilic microbes emphasizing on archaeal and bacterial role.

Potential management for neurodegeneration
Autism Spectrum Disease (ASD) encompasses symptoms like impairment in language, deficit in socialization and hyperactive repetitive stereotypic behaviour. Autism was first defined by Leo Kanner in 1943 as a neurodevelopmental disorder among children [17]. According to United States Centres for Disease Control and Prevention (CDC), approximately 1 in 59 children are diagnosed with ASD [18]. Excessive oxidative stress and lower antioxidant capacity are the two causes of this disease. ASD become one of the most challenging disorders due to lack of successful curative practices till date. The disequilibrium state between prooxidant species and antioxidant defence causes mitochondrial dysfunction. The interconnected pathways of folate, methionine and glutathione metabolism severely impaired which leads to oxidative stress. A dramatic surge occurs in the levels of Reactive oxygen species (ROS) and Reactive nitrogen species (RNS). Brain is the most sensitive organ for oxidative damage caused by ROS because of its constant requirement of oxygen for its function, low antioxidants defences and high content of omega-3 polyunsaturated fatty acids (PUFAs) attributed to be oxidized. Figure 2 depicts the interaction of ROS and brain activity in ASD patients.
The alteration of gut microflora composition with enriched halophilic bacteria has increasing possibilities as a therapeutic approach of disease control [19]. Recent study emphasizes that brain activity is directly linked with gut microbiota [20]. Halophiles maintain metabolic homeostasis by accumulating compatible solutes including quaternary amines (glycine, betaine, ectoine), polyols (arabitol, glycerol, sorbitol) and sugar derivatives (trehalose, sucrose, glucosylglycerol) that manifests immune, enteric, and neural pathways. Thus, multidirectional crosstalk system between gut-brain axis (GBA) modulates the level of oxidative stress by increasing prooxidant components or reduced antioxidant enzymes capacity [21]. Another mechanism involved is the production of short chain fatty acids (SCFAs) by gut colonizing bacteria like Enterococcus, Klebsiella and Staphylococcus. SCFAs are mostly acetate (C 2 ), propionate (C 3 ), and butyrate (C 4 ) compounds produced through carbohydrate fermentation which enters the central nervous system (CNS) by crossing blood-brain barriers and modulate ASD by altering the level of neurotransmitters [22]. The Human Microbiome Project Consortium identified predominantly Firmicutes and Bacteroidetes phyla in gut [23]. There are novel techniques which abandon brain disorders by modulating gut microflora. The discovery of editing technique by clustered regularly interspaced short palindromic repeats (CRISPR) and the associated nuclease 9 has a wide array of possibilities to manipulate the human microbiome [24]. Faecal microbiota transplantation (FMT) therapy with halophilic microorganism could be a promising treatment for autism [25].

Pigment for remote sensing
Halophilic microorganisms produce various types of pigments which protect their delicate cells from external damages. Carotenoid in Deinococcus sp. serve as protective agent against oxidative damage. Red pigment in Rubrobacter radiotolerans itself acts as an effective antioxidant. Dynamic spatial-temporal variations of pigments can be measured through satellite remote sensing system. Specific algorithms are used to process multispectral raw data monitored from satellite sensors. Halophilic archaea, Halobacterium salinarium, produce red colouration due to presence of β-carotene, bacterioruberins (C50 analogues of carotenoids), lycopene and diphytanyl-glycerol pigments. Haloferax volcanii possess bacterioruberin which confers pink hues in Dead Sea, Jordan. Bacterial genera, Salinibacter, thrive in hypersaline lakes and solar salterns with non-photosynthetic retinal pigments, salinixanthin and xanthorhodopsin [26]. Atmospheric circulation pattern of The Great Salt Lake, USA can be estimated by the reflectivity changes of redcoloured patches from the International Space station.
Haloarchaeal retinal (C20) is a bacterioruberins purple coloured pigment, similar to higher plant photosynthetic pigments, a valuable reflectance database from visible to near-infrared region of spectrum produced by chromophore. Dense population of bacterial bloom on ocean surface can be observed by satellite, which reveals thermohaline circulation current [27]. These surface reflected radiation and time-dependent modulations are detectable biosignatures. Purple tinged molecule supposed to be dominant during the Archean  [28]. "Purple Earth Hypothesis" was conceived by Das-Sarma, which said that halophilic bacteria used this purple-tinged retinal molecule to harness energy from the sun [29]. Chlorophyll, the main photosynthetic pigments of plants absorb red and blue wavelength of light and reflects green ones. Chlorophyll molecule may have been preceded into earth from this retinal governed life form in earth (Fig. 3). Today, retinal is found in the plum-coloured membrane of photosynthetic bacteria which absorb green light and give off the purple colour.
Polyextremophilic microorganisms suggest this similar model would be potential biosignature to trace possible biological history away from our planet Earth.

Production of biofuel
Current energy system needs a huge revolution to meet the twenty-first century's demands which must be economically efficient and environment friendly. The fossil fuels are running out at an alarming rate, the depletion is forcing to search for renewable bioenergy as alternative power sources. Widespread implementation of first generation (1G) biofuels is limited due to its high costs and barely developed technology. The second generation (2G) of biofuel based on cellulosic energy is enhancing energy security. Different forms of biofuels are bioethanol, biobutanol, biogas, hydrogen, and biodiesel. Bioethanol is the most suitable substitute among all the biofuels. Halophiles directly involved in ethanol and butanol production by sugar fermentation technique in hypersaline areas around the globe. Nesterenkonia sp. is one of its kind, reported to produce butanol and acetone under aerobic and anerobic fermentation [30]. Fendrich noticed that small amount of ethanol and acetate are produced from gram-positive, obligate halophilic bacterium, Clostridium halophilum in strictly anerobic condition [31]. By undergoing the anaerobic digestion process, halophiles produce large quantities of biogas. Lipase and cellulase from haloarchaeal strain Haloarcula, are being used in biodiesel production [32], it is estimated that 80% of biodiesel production can be achieved successfully from the archaea. Supplementation with ectoine, derived from haloarchaea promote cell growth and 57.1% increase in volumetric ethanol production [33]. Halotolerant bacterial consortia of Joostella marina, Algoriphagus ratkowskyi and Halomonas meridiana can degrade plant based lignocellulosic material which in turn converted into bioethanol by hydrolysis and fermentation [34].

Enzymes from halophilic microorganisms and their industrial applications
The adaptation of protein stability under osmotic pressure, high pH and salinity attracts many industries as best suited candidate for enzymes. Halophiles produce stable enzymes that bring success to all industrial biotechnology goals. Table 1 summarizes some useful enzymes produced by microorganisms under salinity and their potential uses in biocatalyst industries. Numerous hydrolase enzymes have scope to make third generation (3G) biofuel from algal biomass.

Metal bioremediation by nanoparticles
Remediating metal contaminants and waste by biogenic nanoparticles (NPs) from haloarchaea and halobacteria is a potential solution for metal pollution [54]. Halophilic bacteria are resistance to metals due to enzymatic detoxification, extra and intracellular precipitation and energy-dependent efflux system [55]. Table 2 summarizes various nanoparticles synthesized by halophiles. Numerous essential factors like pH, temperature, salt concentration, size of NPs can affect the properties of NPs. Multitude of these factors influence the efficacy of NPs [64]. Reaction media pH played important role in morphology, stability and growth of nanoparticles. It was noted by Mishra et al. that reduction in selenite stopped above pH 8.0 in Bacillus sp. [56]. Metal reduction efficiency attainted maximum at pH.
Concentration of salt (% of NaCl) has a specific effect on the process of NP synthesis from halophilic microorganisms. Selenium nanoparticles synthesized by Bacillus megaterium affected by NaCl concentration in the solution. Decrease in selenite reduction activity above 7% NaCl [56]. Similar observation was noted in Halobacillus sp. showing resistance to CdCl 2 (1.375 mM) at 22% NaCl [58]. This could be due to the negatively charged residues in salt bridges of halophilic protein may involve a multipoint adherence of positively charged residues on the nanoparticle surface which contribute greater stability of the NPs [61].
Nanofabrication process required ambient temperature for successful NP conjugation. Increased temperature above 32 C in graphene oxide led marginal effect in reduction to graphene [57].
The quality and biological applications of NPs depend on particle size. The colour change during AgNPs agglomeration associated with the size of AgNPs in Cupriavidus necator [63]. Raveendran et al. have noticed a shift in the UV absorption peak for ZnS:Mn-Quatum dots due increment of the NP size above 5 nm [60].

Small peptides as potential biomedicine
Antimicrobial peptides (AMPs) are 12-100 amino acids long, α-helical, positively charged, amphiphilic molecules. Halocins are type of antimicrobial peptides, produced naturally by extreme halophilic archaea and released out into the environment. Bacteria produces ribosomally synthesized small peptide molecules (less than 100 amino acid residues), bacteriocin which can inhibit the growth of other bacteria [65]. Bacteriocins exhibit wide range of antimicrobial activity against several antibiotic resistant planktonic bacteria and has broad inhibition spectrum against yeasts, insects and mammals ( Table 3).
The rise of multi drug resistant (MDR) bacterial strains necessitates the search for new platforms for treatment of human diseases. Bacteriocins nowadays are considered as alternative medicine against these microbial pathogens. Class I bacteriocins, also called lantibiotics, possesses post translationally modified unusual amino acid lanthionine. Halophilic bacteria, Staphylococcus simulans produce Nukacin, a variant of bacteriocin, which shows bacteriostatic action to Bacillus subtilis [70]. A two-peptide bacteriocin produced by vancomycin resistant strains of Enterococcus faecalis is active against foodborne pathogen Listeria spp. [71]. Halobacteria produces halocin during transition stage from exponential to stationary phase of growth. H6/H7 type of halocin from Haloferax gibbonsii can inhibit Na + /H + antiporter in mammalian cell. Halocin H6 decreases infarct size during myocardial ischemia and protect reperfusion injury of heart [72]. This finding call renaissance of medical science in terms of organ transplantation. Both H4 and C8 types of halocins from Halobacterium can alter the shape of rod cells into spherical cells in sensitive strains, which leads to cell lysis [73]. Numerous virus-host interactions beyond domain boundaries in hypersaline environment are possible because of halocin. The bacteriocin compound are being used to control infectious bacteria [74]. Halobacterium sp. [35] Haloferax mediteranei [35] Natrial bamagadii [35] Natronomonas pharaonic [35] Pseudoalteromonas sp. 55   Subtilisin-A from Bacillus firmus exhibits anti-microbial (against pathogenic bacteria like Staphylococcus aureus, Corynebacterium diphtheria and Pseudomonas aeruginosa), antifungal (Aspergillus niger, Aspergillus flavus), antioxidant activities (against DPPH and nitric oxide radicals) and anti-cervical cancer activity (against HeLa cell line with IC50 at 53 μg/mL). Thus, bioactive AMPs could be used in therapeutics [75].

Formulation of new generation of liposomes
Liposomes are microscopic vesicular structures consisting of lipid bilayers with an internal fluid compartment which can encapsulate and deliver small molecule drugs or vaccines to a specific target site in the body. Archaeosomes are liposomal formulation made up with ether linked total polar lipids (TPL) of the domain Archaea, conferring high stability of liposomes [76]. Sulfated glycolipid gas vesicles from Halobacterium salinarum elicit both antibody and cell mediated immunity. Isoprenoid glycerolipid vesicles from Methanobrevibacter smithii stimulate the activation of APC mediated expression of both MHC class I and II, induce co-stimulatory molecules and make cytotoxic responses to CD8 + cell. Both macrophage and dendritic cell evoke a memory generation even in the absence of CD4 + helper T lymphocytes [77]. Higa et al. developed ultradeformable liposome from Halorubrum tebenquichense which can enhance skin permeation ability and could be carrier of transdermal delivery for procyanidins [78]. The rapid, low-cost drug delivery approach represents haloarchaea an excellent candidate for treating allergies, cancer and neoplastic diseases as nano-delivery based vaccination. But a major limitation of liposome mediated drug delivery lies in quality assurance and cost. Sometime phospholipid of liposome hydrolyzed by protease. Future research will overcome the existing challenges and surpass the current limitations.

Repairment of concrete crack caused by road salt
Snow and ice removal is one of the most crucial things in winter to keep moving road traffic in the coldest countries in the world. Anti-icing brine solution containing sodium chloride, magnesium chloride and calcium chloride are applied to combat icing period. The application of de-icing road salt began in the 1930s and it became widespread in highway maintenance during the 1960s [79]. It has been reported that 20 million tons of highway salts were being used in the year 2016 in USA only [80]. NaCl is used mostly as anti-freeze because it is the least expensive and easily available and works at temperatures above − 12 °C. Road salt run-off has created severe ecological changes and affected the soil microflora in many countries. Salt-laden water interact with root systems and produce adverse effects in local vegetation. Halophilic bacteria are able to survive in high pH (pH 9.0) and grows in negative osmotic potential. Few studies have been reported the impact of excess salt in microbial communities in terms of soil fertility. Archaeal genera, Natrinema and Haloterrigena are known for soil stabilization [81]. Figure 4 shows halophilic bacteria Sporosarcina pasteurii plays fundamental role in biomineralization by converting calcium ions from dumped road soil by urease enzyme into calcite or calcium carbonate also known as limestone. This bacterium aided in biocementing the cracks in concrete by depositing CaCO 3 precipitation [82]. Halophilic bacteria, Pseudomonas putida and Vibrio fischeri (standard bacterial test) have been used to estimate the toxicity level and the physical and chemical properties of soil [83]. As a permanent member of the microbiota in de-icing salts, halophilic bacteria plays a central role in soil ecosystems.

Waste management by biodegradation of hydrocarbon and plastics
Halophiles are involved in biodegradation of wide range of hydrocarbons. Biological treatment of saline wastewater by heterotrophic, halotolerant microbes catches tremendous demand in industrial scale. Basic evolutionary study suggests oldest halophilic microbial diversity found in stromatolite during Precambrian period resemble modern microbial mat growing in hypersaline lakes [84]. Marinobacter is a metabolically versatile hydrocarbon degrading taxon. Halotolerant bacteria, Alcanivorax, Burkholderia, Pandoraea and Enterobacter can degrade broad spectrum of petroleum hydrocarbons like benzene, ethylbenzene, n-alkanes (C6-C40) compounds and xylene into carbon dioxide [85]. Salt tolerant bacteria, Pseudomonas putida and Ralstonia pickettii predominantly chemotactically swim towards toluene and mineralize benzene and phenol. Archaeal strain, Haloferax, Halobacterium, and Halococcus from hypersaline Gulf area use benzoic acid salicylate as sole carbon and energy source and converted to catechol under aerobic condition. Biotransformation of hydrocarbon produce several higher-value intermediate products like benzyl alcohol, phenolic compounds and catechol. Potent natural antioxidant hydroxytyrosol (HTyr) produced by Halomonas sp. strain HTB 24, is isolated from hypersaline olive mill wastewater. Pseudomonas aeruginosa, isolated from crude oil contaminated soil can transform polyphenolic compound 4-tyrosol to HTyr, which has well documented anti-inflammatory activity [86]. Another most formidable environmental challenge today is plastic waste. The non-degradability of plastics and petrochemical-derived polymers are aggravated by extensive global use and their inaccurate management [87]. Microplastics (< 5 mm in size) are ubiquitous from deepest ocean floor to highest mountains, very recently in human blood [88].
Polyhydroxyalkanoates (PHA) are natural linear polyester that are produced by microbial fermentation processes, having similar thermochemical properties like molecular weight, semicrystallinity, piezoelectricity, low permeability for water and oxygen, melting temperature (T m ) with the additional advantage of being completely biodegradable [89]. PHA synthesized by several haloarchaea and halophilic bacteria when there is availability of excess carbon sources (substrate) and limited oxygen, nitrogen supply [90]. Among the different classes of PHA's like poly-3-hydroxybutyrate (PHB), poly(3hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), polyhydroxyvalerate (PHV), PHBs are only 100% biodegradable ones, thus offer the best solution to protect environment from plastic pollution [89]. During intracellular degradation, 3PHB is oxidized by dehydrogenase and converted into acetyl-CoA by β-ketothiolase to form non -toxic substances.
A number of Gram positive and Gram negative nonhalophile PHA producing bacteria have been reported. Among them, Pseudomonas putida, Burkholderia thailandensis, Alcaligenes latus, Escherichia coli are gaining interest due to high yield of PHA and substrate spectrum [91]. But PHA production from halophilic microorganisms have added benefits due to its low -cost purification process and minimal environmental hazards. After fermentation, downstream processing of PHA plays important role in production cost. Intracellularly produced PHA granules from halophiles can be done by osmotic lysis that burst the particles and separated easily from non-PHA cell mass (NPCM) [91]. Halophiles can utilize cheap and renewable carbon sources for PHA production. A brief summary of some important PHA producing halophiles is given in Table 4.

Bioremediation for sustainable agriculture
Rising soil salinity is a global concern for crop production. Salinity affects all aspects of plant development in

Future prospects
The search for new life elsewhere in the galaxy has gained an increasing interest over the past two decades. Astrobiologists detected most promising sign of life on the extrasolar planet, Mars, called exoplanet biosignature [101]. Data collected from ExoMars Mission (2018) confirms that Mars temporarily hosted abundant water on the surface [102]. Salty water pockets and frigid water acquirers near South Martian poles are packaged with manganese and calcium perchlorate which is most likely to be habitable for halophilic bacteria [103]. Molecular structure resembles that haloarchaeon, Halorubrum chaoviator, could survive in freezing and thawing brine in Mars [104]. The archaea with higher tolerance to perchlorate (upto 0.4 M), can use chlorate as terminal electron acceptor during anaerobic respiration [105]. Therefore, relative abundance of perchlorate in the Martian regolith could be analogues for understanding nitrogen fixation on extremely arid regions, like Atacama Desert on Earth. These investigations pointed that emergence and adaptation of life in salt brine during prebiotic evolution could be the earliest signature of life. Thus, halophiles could be used in future for searching extra-terrestrial life.

Conclusions
The study of halophillicity of the microorganisms provides significant insight as the basis of potential biotechnological applications. The availability of modern techniques of bioengineering and molecular biology would enable to construct biomolecules for industrial interest. Existence of life in Earth like planets though speculative, plausible model of halophilic life has been correlated to great extent with available data, provides substantial hope for identifying new ways of research in Astrobiology. The discovery and progressive characterization of halophiles in gut-brain research have created new clinical therapies for neuro degenerative disorders. The ability of these life forms to withstand high salt concentration, osmotic pressure, pH and often a combination of these conditions, made a transforming insight to use them as a promising candidate in various industries. Moreover, production of biofuel, bioremediation of synthetic compounds and biocleaning of environment have crucial values for several green purposes in maintaining the environmental sustainability.