Table 1 Summary of purification profile of bacteriocin ABC transporter ATP-binding protein produced by Enterococcus casseliflavus MI001 strain
Type of sample | Volume collected (ml) | Total protein (mg) | Total activity (AU) | Specific activity (AU/mg) | Fold purification |
|---|---|---|---|---|---|
Crude | 150 | 129 | 240,000 | 1860 | 1 |
Ammonium sulphate precipitation and dialysis | 10 | 3.6 | 18,000 | 5000 | 2.688 |
Gel filtration chromatography (SephadexG-75) | 5 | 1.2 | 11,000 | 9166 | 4.927 |
Ion exchange chromatography (DEAE cellulose) | 2 | 0.32 | 4800 | 15,000 | 8.064 |