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Table 2 Morphological, physiological, and biochemical properties and amino acid utilization of Streptomyces sp. SBLWN_MH2

From: New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

Characteristics

Results

Morphological characteristics

 Spore chains

Spiral

 Spore surface

Smooth

 Color of aerial mycelium

White series

Physiological characteristics

 Melanin production

Positive

 Action of milk

No coagulation in 14 days

 Nitrate reduction

Positive

 Gelatin liquefaction

Negative

 Starch hydrolysis

Positive

 Cell wall

ll-diaminopimelic acid

Carbon utilization

 No carbon

d-glucose

+

d-xylose

±

l-arabinose

d-fructose

 Raffinose

d-mannitol

+

 Meso-inositol

±

 Salicin

ND

 Sucrose

±

Temperature tolerance (°C)

 28–30 °C

Abundant

 40 °C

Weak

 50 °C

No growth

Amino acids

dl-methionine

dl-iso-leucine

+

l-arginine

+

l-lysine

l-glutamic

l-histidine

+

 Ph-alanine

+

l-aspargine

+

l-valine

+

l-cysteine

+

 Glycine

++

 Prolene

+

 Ornithine

+

 Tyrosine

+

dl-serine

+

NaCl % resistance

 0

Gray, abundant

 3–5

White, good

 7–10

Light gray, weak

 13

No growth

Antibiotic susceptibility (mm)

 Rifamycin (RD, 5 μg)

14b

 Vancomycin (VA, 30 μg)

40c

 Streptomycin (S, 10 μg)

00a

 Neomycin (N, 30 μg)

30c

 Tetracycline (TE, 5 μg)

20c

 Nalidixic acid (NA, 30 μg)

15b

 Novobiocin (NV, 30 μg)

40c

Cefodizime (CDZ 30 μg)

00a

  1. ND not detected
  2. “+++” = good growth, “++” = moderate growth, “+” = weak growth, “−” = negative
  3. aSusceptible
  4. bIntermediate
  5. cResistant