Table 6 The optimum parameters and lipase productivity defined by the Box-Behnken design and RSM
Fermentation parameter | Amount |
|---|---|
Parameters for maximum lipase production | |
Soluble starch conc. (g/l) | 1.00 |
Casein (g/l) | 0.30 |
K2HPO4 (g/l) | 2.00 |
KNO3 (g/l) | 2.00 |
NaCl (g/l) | 50.00 |
MgSO4.7H2O conc. (g/l) | 0.05 |
FeSO4.7H2O (g/l) | 0.01 |
Anhydrous CaCO3 (g/l) | 0.02 |
Linseed oil (%) | 1.00 |
Tween-80 conc. (g/l) | 4.00 |
Medium pH | 8.20 |
Time course (day) | 3.00 |
Lipase productivity (U/ml) | |
Yield | 26.54 |
SE mean | 03.25 |
95% CI low | 19.04 |
95% CI high | 34.05 |
SE Pred | 04.41 |