From: Characterization of squalene synthase gene from Gymnema sylvestre R. Br.
Designed from | Name of the primer | Sequences | Product size | GC% | Tm | Corresponding lane on gel | |
---|---|---|---|---|---|---|---|
GS-SQS CDS 10191 | GS-SQSLR | F | ATGGGGAGCTTGGGAACCAT | 6.2 k | 55 | 53.8 | Fig. 4, lane 2 |
GS-SQSLR | R | TCACATATCAATTGGTCGATTGGC | 42 | 54.0 | |||
GS-SQS scaffold_3347 | GS-SQSINT1 | F | ATGGGTTTGTTTCTACAG | 144 bp | 39 | 49.3 | Fig. 7, lane 2 |
GS-SQSINT1 | R | GTCTTCCAGATA ATCCCTAATGATG | 40 | 62.0 | |||
GS-SQSINT2 | F | GGAGCAAATATGTCAATAAACTTGAGG | 217 bp | 37 | 63.7 | Fig. 7, lane 6 | |
GS-SQSINT2 | R | GACAGAATTCTCCTCGTATTTTAAGT | 35 | 61.7 | |||
GS-SQSINT1 | F | ATGGGTTTGTTTCTACAG | 415 bp | 39 | 49.3 | Fig. 7, lane 10 | |
GS-SQSINT2 | R | GACAGAATTCTCCTCGTATTTTAAGT | |||||
Housekeeping gene | EFTU F | F | AGGTCCACCAACCTTGACTG | 200 bp | 55 | 60.00 | Fig. 7, lane 14, 16 |
EFTU R | R | GGAGCAAAGGTCACAACCAT | 50 | 60.00 |