Characterization of squalene synthase gene from Gymnema sylvestre R. Br.

Beni-Suef University Journal of Basic and Applied Sciences

Table 1 Details of primers (5′–> 3′) used in the study

Designed from	Name of the primer		*Sequences*	Product size	GC%	Tm	Corresponding lane on gel
GS-SQS CDS 10191	GS-SQSLR	F	ATGGGGAGCTTGGGAACCAT	6.2 k	55	53.8	Fig. 4, lane 2
GS-SQS CDS 10191	GS-SQSLR	R	TCACATATCAATTGGTCGATTGGC	6.2 k	42	54.0	Fig. 4, lane 2
GS-SQS scaffold_3347	GS-SQSINT1	F	ATGGGTTTGTTTCTACAG	144 bp	39	49.3	Fig. 7, lane 2
	GS-SQSINT1	R	GTCTTCCAGATA ATCCCTAATGATG	144 bp	40	62.0	Fig. 7, lane 2
	GS-SQSINT2	F	GGAGCAAATATGTCAATAAACTTGAGG	217 bp	37	63.7	Fig. 7, lane 6
	GS-SQSINT2	R	GACAGAATTCTCCTCGTATTTTAAGT	217 bp	35	61.7	Fig. 7, lane 6
	GS-SQSINT1	F	ATGGGTTTGTTTCTACAG	415 bp	39	49.3	Fig. 7, lane 10
	GS-SQSINT2	R	GACAGAATTCTCCTCGTATTTTAAGT	415 bp			Fig. 7, lane 10
Housekeeping gene	EFTU F	F	AGGTCCACCAACCTTGACTG	200 bp	55	60.00	Fig. 7, lane 14, 16
Housekeeping gene	EFTU R	R	GGAGCAAAGGTCACAACCAT	200 bp	50	60.00	Fig. 7, lane 14, 16