In situ green analytical methods for the rapid and sensitive determination of a newly launched orphan anticancer drug; Tigecycline in infusion bags: comparative study

Beni-Suef University Journal of Basic and Applied Sciences

Table 3 Intra-day and inter-day precision and accuracy for the determination of TIG in intravenous infusion solution using the proposed methods

Method	Conc. (µg/mL)	Average % recovery ± SD^a	% RSD^b	% E_r^c
(a) Accuracy and intra-day precision
CZE method	20	99.85 ± 1.52	1.52	− 0.15
	100	99.23 ± 1.79	1.80	− 0.77
	300	99.47 ± 0.34	0.34	− 0.53
Spectrophotometric methods
Method I	40	101.37 ± 0.42	0.42	1.37
	100	98.60 ± 0.37	0.37	− 1.39
	200	101.84 ± 0.37	0.37	1.84
Method IIA	40	100.85 ± 1.44	1.43	0.85
	80	101.06 ± 1.55	1.53	1.06
	140	98.61 ± 0.33	0.33	− 1.39
Method IIB	40	101.44 ± 0.85	0.83	1.44
	80	101.82 ± 0.76	0.75	1.82
	140	100.18 ± 0.61	0.61	0.18
Method III	20	100.38 ± 1.55	1.54	0.38
	80	100.67 ± 0.46	0.46	0.67
	140	99.38 ± 0.05	0.05	− 0.62
(b) Accuracy and inter-day precision
CZE method	20	101.18 ± 0.99	0.98	1.18
	100	99.03 ± 1.97	1.99	− 0.97
	300	98.78 ± 0.99	1.01	− 1.22
Spectrophotometric methods
Method I	40	101.86 ± 0.42	0.41	1.86
	100	98.35 ± 0.56	0.57	− 1.65
	200	101.60 ± 0.28	0.27	1.60
Method IIA	40	98.01 ± 1.93	1.96	− 1.99
	80	100.92 ± 1.96	1.94	0.92
	140	98.65 ± 0.19	0.19	− 1.35
Method IIB	40	99.58 ± 0.90	0.91	− 0.42
	80	98.75 ± 1.41	1.43	− 1.25
	140	100.14 ± 0.18	0.18	0.14
Method III	20	98.63 ± 0.57	0.58	− 1.37
	80	99.41 ± 1.41	1.42	− 0.59
	140	98.49 ± 1.18	1.19	− 1.51