Preparation and characterization of polymyxin B- and histidine-coupled magnetic nanoparticles for purification of biologics from acquired endotoxin contamination

Beni-Suef University Journal of Basic and Applied Sciences

Table 1 Fractional factorial design for optimization of preparation of MNPs with results showing the selected experiment (run order 14)

Run order	pH	Temp	Molarity of iron salts	Dextran%	Time	Ammonia%	z-average	PdI	pk1 area intensity%
1	11	60	2:1	20	15	28	51.26	0.467	91.3
2	9	60	2:1	20	60	28	89.71	0.302	93.8
3	11	85	2:1	50	60	28	54.16	0.376	52.5
4	11	85	1:1	20	15	28	82.66	0.643	83.1
5	11	85	1:1	50	15	7.5	104.8	0.371	91.7
6	10	72.5	1:1	35	37.5	17.75	117.6	0.249	100
7	11	60	1:1	50	60	28	70.22	0.27	97.1
8	11	60	2:1	50	15	7.5	92.38	0.26	100
9	10	72.5	2:1	35	37.5	17.75	81.87	0.264	100
10	9	85	2:1	20	15	7.5	89.53	0.308	100
11	9	60	1:1	50	15	28	104	0.229	99
12	9	60	1:1	20	15	7.5	90.15	0.358	85.1
13	9	85	1:1	20	60	28	99.7	0.401	98.8
14	9	85	2:1	50	15	28	59.04	0.275	98.2
15	11	85	2:1	20	60	7.5	493.4	0.533	58.7
16	11	60	1:1	20	60	7.5	115	0.205	100
17	9	85	1:1	50	60	7.5	96.06	0.28	98.8
18	9	60	2:1	50	60	7.5	65.74	0.257	95