Fig. 5

Photomicrograph of hematoxylin and eosin stained liver section from a: control group showing normal central vein (CV) in the middle, hepatocytes (H), adjacent sinusoids (S) which contain Kupffer (K) cell. b1: DMBA-treated group showing congested central vein (CV), severe degeneration in hepatocytes with vacuolated cytoplasm (V) and pyknotic nuclei (arrow). b2: Showing severe vacuolated cytoplasm (V), severe degenerated hepatocytes, pyknotic (black arrow) karyorrhexes (zigzag arrow), darkly stained Kupffer cells (K). b3: Showing portal vein (PV) with thickened wall surrounded by fibrosis (curved arrows) and proliferated bile ductules (Bc). c: DMBA + Art-treated group showing dilated central vein (Cv), blood sinusoids containing dark staining Kupffer (K), some hepatocytes with pyknotic nuclei (black arrow), karyorrhexes (zigzag arrows) and other hepatocytes with karyomegaly (arrow heads). d: DMBA + Ech-treated group showing liver cells with few cytoplasmic vacuolation (V), few hepatocytes appeared with pyknotic nuclei (black arrow) or karyolyzed (zigzag arrows) and hypertrophied Kupffer (K) cells. Some binucleated nuclei (Bi) were seen. e: Art + DMBA-treated group showing some improvements in histological architecture of hepatocytes with nearly normal nuclei (N) and others with binucleated nuclei (Bi), normal portal vein (pv), blood sinusoids (S) and Kupffer (K) cell with few cytoplasmic vacuolations (V). f: DMBA + Ech-treated group showing nearly normal histological architecture of hepatocytes with normal central (Cv) and hypertrophied Kupffer cells (K) in blood sinusoids (S) except few pyknotic nuclei (black arrow) and some karyolized nuclei (zigzag arrow) and binucleated (Bi) hepatocytes (Hematoxylin and Eosin, Scale bar = 20 μm)