Table 5 The nucleotide sequences and the annealing temperature of the SSR primers
No | Primer name | Forward (5ʹ–3ʹ) | Reverse (5ʹ–3ʹ) | Annealing Temp.℃ |
|---|---|---|---|---|
1 | BRMS01 (GA)25 | GGTGGCTCTAATTCCTCTGA | ATCTTTCTCTCACCAACCCC | 53 |
2 | BRMS02 (CT)22 | GATCTTCTCTCCAAAA | TCCAAGCTAAATTACG | 45 |
3 | 3 BRMS03 (CT)19 F | ACGAATTGAATTGGACAGAG | CAGATGGGAGTCAAGTCAAC | 51 |
4 | A049627743 | ATGGAATCTGCTCATCTCAC | TAAGCTGCAATGATCAAAGAT | 49 |
5 | A0415440685 | TTTGAACGATACACAACAACA | GTTGGTCCACGAGTAAAAGAT | 52 |
6 | A05 20,242,013 | AGAAGCTTTTTCTCTTGTTGC | TGATGTAAGAGCGTGAAAGAT | 50 |
7 | A01-21,437,996 | GAATACATGGAGAGATCTGGA | CATTTAGAAATCAGAGCGAAG | 51 |
8 | BG 103 | TTTGTCCACCATTTCTTAAACATCTA | TCAATGAAATTGTTAAAATACAGCAA | 53 |
9 | BG 1 | GCTGGCTGCACAATAACAGA | TACCACTGGAGGAGCTTCG | 55 |
10 | BG 55 | TCCCAAGTGGTGCTCCTTT | GGTAATCCCTTTTCTGCAAGC | 53 |
11 | BRMS56 (GA)13 | GATCAAGGCTACGGAGAGAGAG | CGTGACGCTAGAGTAATCGAGT | 58 |
12 | BRMS17 (CA)33 | GGAAAGGGAAGCTTCATATC | CTGGAAAGCATACACTTTGG | 51 |
13 | SR1 | GTTTGGTTCAGAGGCAGAGG | CTATCGCTGCAGAAGAAGGG | 55 |
14 | BJ 96 | GATCTTCTCTCCAAAACTCTCT | AAAGTCCAAGCTAAATTACAAA | 50 |
15 | BJ 95 | CGTAAGTTTCAATTGTCAACGG | TCGTACGAAACAATCAACGG | 52 |
16 | IM 4 | CCCAAACGCTTTTGACACAT | GGCACAATCCACTCAGCTTT | 52 |
17 | IM 8 | GCGATGTTTTTTCTTCAGTGTC | TTAATCCCTACCCACAATTTCC | 53 |
18 | SR 37 | TATGTACACATTCCTCATTTTC | CATTCGTCTCCACCTTCT | 49 |
19 | BRMS19 (GA)10 | CCCAAACGCTTTTGACACAT | GGCACAATCCACTCAGCTTT | 52 |
20 | BRMS27 (GA)17 | GTGCTTGATGAGTTTCACATTG | GCAGGCGTTGCCTTTATGTA | 53 |