Table 1 Primer sequences and sizes of expected amplicons of each PCR assay
From: Carbapenemase producing Enterobacterales clinical isolates from a tertiary care hospital in Egypt
Target gene | Primers (5′–3′) | Size of amplicon (bp) | Annealing temp | References |
|---|---|---|---|---|
blaVIM | F: GATGGTGTTTGGTCGCATA R: CGAATGCGCAGCACCAG | 390 | 52 °C | [14] |
blaIMP | F: GGAATAGAGTGGCTTAAYTCTC R: GGTTTAAYAAAACAACCACC | 232 | 52 °C | [15] |
blaNDM | F: GGTTTGGCGATCTGGTTTTC R: CGGAATGGCTCATCACGATC | 621 | 52 °C | [14] |
blaOXA-48 | F: GCGTGGTTAAGGATGAACAC R: CATCAAGTTCAACCCAACCG | 438 | 52 °C | [15] |
blaKPC | F: CGTCTAGTTCTGCTGTGTTG R: CTTGTCATCCTTGTTAGGCG | 798 | 52 °C | [15] |