SRAP and IRAP revealed molecular characterization and genetic relationships among cowpea (Vigna unguiculata L.) irradiated by gamma-ray

Beni-Suef University Journal of Basic and Applied Sciences

Table 1 SRAP combinations used in the PCR reaction and their sequences, GC%, and annealing temperature

No.	Primer			GC%	Ann. temp
1	SRAP-ME3EM1	F	ME3-5′-TGAGTCCAAACCGGAAT-3′	47	45
1	SRAP-ME3EM1	R	EM1-5′-GACTGCGTACGAATTAAT-3	39	45
2	SRAP-ME4EM1	F	ME4-5′-TGAGTCCAAACCGGACC-3′	59	49
2	SRAP-ME4EM1	R	EM1-5′-GACTGCGTACGAATTAAT-3	39	45
3	SRAP-F12R9	F	F12-5′-CGAATCTTAGCCGGAGC-3′	59	49
3	SRAP-F12R9	R	R9-5′-GACACCGTACGAATTTGA-3′	44	47
4	SRAP-ME1EM2	F	ME1-5′-TGAGTCCAAACCGGATA-3′	47	45
4	SRAP-ME1EM2	R	EM2-5′-GACTGCGTACGAATTTGC-3′	50	49
5	SRAP-F13R15	F	F13-5'-CGAATCTTAGCCGGCAC-3'	59	49
5	SRAP-F13R15	R	R15-5′-CGCACGTCCGTAATTCCA-3′	56	51
6	SRAP-ME5EM2	F	ME5-5′-TGAGTCCAAACCGGAAG-3′	53	47
6	SRAP-ME5EM2	R	EM2-5′-GACTGCGTACGAATTTGC-3′	50	49
7	SRAP-F13em1	F	F13-5'-CGAATCTTAGCCGGCAC-3'	59	49
7	SRAP-F13em1	R	EM1-5′-GACTGCGTACGAATTAAT-3	39	45