Table 2 IRAP combinations used in the PCR reaction and their sequences, GC%, and annealing temperature
No. | Primer name | Sequence | GC% | Ann. temp | |
|---|---|---|---|---|---|
1 | IRAP 2175 | 5′-TTAGACCCGGAACCGCCGTG-3′ | 65 | 61 | |
2 | IRAP 2198 | 5′-ATCCTTCGCGTAGATCAAGCGCCA-3′ | 54 | 69 | |
3 | IRAP 2197 | 5′-GAAGTACCGATTTACTTCCGTGTA-3′ | 42 | 63 | |
4 | IRAP 2200 | 5′-ATGTGACAGTCGACTAACCAC-3′ | 48 | 57 | |
5 | IRAP 2202 | 5′-TGGCGCTTGATCTACGCGAAGGA-3′ | 57 | 67 | |
6 | IRAP 2204 | 5′-AACTTGATCCAGATCATCTCC-3′ | 43 | 55 | |
7 | LTRM | F | 5′-GCGCTTAGCGTTAGGCTAACT-3′ | 52 | 59 |
R | 5′-CGTGTAGCCTCTTTGGCCCTA-3′ | 57 | 61 | ||
8 | LTRG3 | F | 5′-CGAGTAGTAGGAAGGAACCGG-3′ | 57 | 61 |
R | 5′-GGCGGCTAGCTTATAGGACTT-3′ | 52 | 59 | ||
9 | CL22 | F | 5′-TGATCAGAGAAGAAAGGGGA-3′ | 45 | 53 |
R | 5′-CACGCAGAGAGATTGACACG-3′ | 55 | 57 | ||
10 | CL34 | F | 5′-GAACGATTACCTCACAGACA-3 | 45 | 53 |
R | 5′-GAGCAATAAAGAGAAGCCCG-3 | 50 | 55 | ||