SRAP and IRAP revealed molecular characterization and genetic relationships among cowpea (Vigna unguiculata L.) irradiated by gamma-ray

Beni-Suef University Journal of Basic and Applied Sciences

Table 6 Band size, polymorphism percentage, allele frequency, and the polymorphism information contents of cowpea revealed by 10 IRAP primers

Ser	Primer	Size (bp)	Total bands	PB	MB	PM%	f	PIC
M₁
1	IRAP 2175	186–2525	28	26	2	92.86	0.36	0.46
2	IRAP 2198	213–1021	27	23	4	85.19	0.45	0.50
3	IRAP 2197	200–2447	19	18	1	94.74	0.46	0.50
4	IRAP 2200	269–2120	28	25	3	89.29	0.33	0.44
5	IRAP 2202	213–517	21	18	3	85.71	0.44	0.49
6	IRAP 2204	224–641	20	16	4	80.00	0.43	0.49
7	LTRM	246–509	27	23	4	85.19	0.40	0.48
8	LTRG3	288–2335	19	15	4	78.95	0.40	0.48
9	CL22	241–1032	26	23	3	88.46	0.35	0.45
10	CL34	250–793	24	22	2	91.67	0.41	0.48
	Total M₁		239	209	30
	Average		24	21	3	87.20	0.40	0.48
M₂
1	IRAP 2175	186–2525	26	23	3	88.46	0.38	0.47
2	IRAP 2198	213–1021	16	15	1	93.75	0.29	0.41
3	IRAP 2197	200–2447	24	22	2	91.67	0.35	0.46
4	IRAP 2200	269–2120	18	18	0	100.00	0.41	0.48
5	IRAP 2202	213–517	20	15	5	75.00	0.46	0.50
6	IRAP 2204	224–641	21	16	5	76.19	0.40	0.48
7	LTRM	246–509	24	24	0	100.00	0.30	0.42
8	LTRG3	288–2335	19	16	3	84.21	0.42	0.49
9	CL22	241–1032	25	24	1	96.00	0.28	0.40
10	CL34	250–793	18	16	2	88.89	0.32	0.44
	Total M₂		211	189	22
	Average		21	19	2	89.42	0.36	0.45
	Total		450	398	52

PB Polymorphic bands, MB Monomorphic bands, PM% Polymorphism percentage, f Allele frequency, PIC Polymorphism information contents