Table 1 Primer sequence of leptin and IGFI genes

Leptin

5'-GCTCCACCCTCTCCTGAGTTTGTCC-3'

5'-TGTCCTGTAGAGACCCCTGTAGCCG-3'

KJ918739.1

65/30 s

290bp

1 cycle at 94 ◦C/3 min., 35 cycles at 95 ◦C/30 s.,72 ◦C/ 30 s., and final extension at 72 ◦C/ 10 min

IGFI

5′-ACTGACCTCATGCGCATGTGCTGG-3′

5′-CTCGTTCTTGCGGCCCCCGTTCAT-3′

XM_027957015.2

52/30

sec

345bp