Table 2 The primers used for PCR and sequencing
From: Comparison of four DNA barcoding loci to distinguish between some Apiaceae family species
Locus | Primer | Sequence 5′–3′ | Amplicon size | Amplification protocol |
|---|---|---|---|---|
rbcL | RbcL_F | CGGTAGCTGCCGAATCTTCT | 533bp | 94 °C 3 min; 94 °C 15 s, 55 °C 25 s, 72 °C 45 s, 30 cycles; 72 °C 5 min |
| Â | RbcL_R | ACCTGTTTCAGCCTGTGCTT | Â | Â |
matK | MatK_F | ACTAATACCCTACCCAGCCCAT | 780bp | Â |
| Â | MatK_R | CTGCAGATACGCCCAAATCG | Â | Â |
trnH-psbA | trnH-psbA.F | ACAAATGGGTAAGGCCGGG | 250,158bp | Â |
| Â | trnH-psbA.R | ACTTGGCTACATCCGCCC | Â | Â |
ITS | ITS1.F′ | TCCGTAGGTGAACCTGCGG | 750bp |  |
| Â | ITS4.R | TCCTCCGCTTATTGATATGC | Â | Â |