Table 1 Primers sequences used for qRT-PCR
Gene symbol | Gene description | Accession number | Primer sequence |
|---|---|---|---|
NF-κB | Nuclear factor kappa-B | NM_001276711.1 | F: 5′‐CACTGTCAACAGATGGCCC‐3′ R: 5′‐GTCTGTGAGTTGCCGGTCTC‐3′ |
TNF-α | Tumor necrosis factor alpha | NM_012675.3 | F: 5′‐ACACACGAGACGCTGAAGTA-3′ R: 5′‐GGAACAGTCTGGGAAGCTCT-3′ |
IL-1β | Interleukin-1 beta | NM_031512.2 | F: 5′-TTGAGTCTGCACAGTTCCCC-3′ R: 5′-GTCCTGGGGAAGGCATTAGG‐3′ |
c-Jun | c-Jun N-terminal kinases (JNKs) | NM_053829.2 | F: 5′-GTCATTCTCGGCATGGGCTA ‐3′ R: 5′-TGGACGCATCTATCACCAGC‐3′ |
c-Fos | Fos proto-oncogene, AP-1 transcription factor subunit | NM_022197.2 | F: 5′‐ACGACCATGATGTTCTCGGG ‐3′ R: 5′‐ GCTGTCACCGTGGGGATAAA‐3′ |
c-Myc | c-myelocytomatosis oncogene product or MYC proto-oncogene, bHLH transcription factor | NM_012603.2 | F: 5′‐AGTCAGGGTCATCCCCATCA‐3′ R: 5′‐ AAAGCTACGCTTCAGCTCGT ‐3′ |
GAPDH | Glyceraldehyde3-phosphate dehydrogenase | NM_017008.4 | F:-5′-ACCACAGTCCATGCCATCAC-3′ R:-5′-TCCACCACCCTGTTGCTGTA-3′ |