Phenolic-rich fraction of green tea attenuates histamine-mediated cardiopulmonary toxicity by inhibiting Cox-2/NF-κB signaling pathway and regulating oxidant/antioxidant balance

Hassanen, Eman I.; Kamel, Shaimaa; Issa, Marwa Y.; Mohamed, Wafaa A.; Mansour, Hayam A.; Mahmoud, Mahmoud A.

doi:10.1186/s43088-024-00464-2

Beni-Suef University Journal of Basic and Applied Sciences

Table 2 Peak assignments of metabolites in the phenolic-rich fraction of Camellia sinensis using UPLC-MS in negative ionization mode

From: Phenolic-rich fraction of green tea attenuates histamine-mediated cardiopulmonary toxicity by inhibiting Cox-2/NF-κB signaling pathway and regulating oxidant/antioxidant balance

Peak no	Assignment	Molecular Formula	RT (min)	Precursor ion m/z [M-H]^‒	Product ions MS/MS	Chemical class	References
1	Malic acid	C₄H₆O₅	0.75	133.0137	115,113,105,89, 87,73,71,57	Organic acid	Liu et al. [54]
2	Citramalic acid/cinnamic acid	C₅H₈O₅	0.77	147.0267	101,85	Organic acid	Liu et al. [54]
3	Oxoadipic acid	C₆H₈O₅	0.79	159.0712	131,115,113,103	Organic acid	Liu et al. [54]
4	(Iso)citric acid	C₆H₈O₇	0.81	191.0226	173,111,87,129, 85	Organic acid	Ezzat et al. [23]
5	Quinic acid	C₇H₁₂O₆	0.83	191.0459	171,127,111	Organic acid	Jeszka-Skowron et al. [39]
6	Caffeoyl-hexoside	C₁₅H₁₈O₉	0.91	341.0954	179,161	Hydrocinnamic acid derivative	Ezzat et al. [23]
7	Quinic acid derivative	C₁₉H₃₄O₁₇	0.94	533.1731	191	Quinic acid derivative	Han et al. [27]
8	Gallic acid	C₇H₆O₅	1.03	169.0139	125,107,97,79	Hydroxybenzoic acid	Hasim Kelebek [45]
9	5-O-Galloylquinic acid (Theogallin)	C₁₄H₁₆O₁₀	1.05	343.1224	191,169	Hydroxybenzoic acid	Han et al. [27]
10	Itaconic acid	C₅H₆O₄	1.11	129.0090	85	Organic acid	Hassanen et al. [31]
11	(Epi)gallocatechin I	C₁₅H₁₄O₇	1.18	305.0819	179,167,137,125	Flavan-3-ol	Shevchuk et al. [80]
12	(Epi)gallocatechin II	C₁₅H₁₄O₇	1.56	305.0814	179,167,137,125	Flavan-3-ol	Shevchuk et al. [80]
13	p-Coumaroylquinic acid I	C₁₆H₁₈O₈	1.79	337.1488	191,163,119	Hydrocinnamic acid	Han et al. [27]
14	(Epi)catechin	C₁₅H₁₄O₆	1.99	289.0767	245,205	Flavan-3-ol	Kelebek [45]
15	Caffeoylquinic acid	C₁₆H₁₈O₉	2.06	353.1350	191,179,135	Hydrocinnamic acid derivative	Wen et al. [95]
16	Coumaroyl hexoside	C₁₅H₁₈O₈	2.12	325.1510	163	Hydrocinnamic acid derivative	Abu-Reidah et al. [2]
17	Isopropylmalic acid	C₇H₁₂O₅	2.22	175.0398	157,131,115,87	Organic acid	Liu et al. [54]
18	Hydroxybenzoic acid	C₇H₆O₃	2.29	137.0280	93	Hydroxybenzoic acid	Jeszka-Skowron et al. [39]
19	(Epi)catechin-(epi)catechin (Procyanidin B) I	C₃₀H₂₅O₁₂	2.48	577.1374	451,425,289,125	Flavan-3-ol	Abu-Reidah et al. [2]
20	Dihydroxybenzoic acid hexoside	C₁₃H₁₆O₉	2.85	315.0737	153,109	Hydroxybenzoic acid	Abu-Reidah et al. [2]
21	(Epi)catechin	C₁₅H₁₄O₆	3.24	289.0810	245,205,179,109	Flavan-3-ol	Kelebek [45]
22	p-Coumaroylquinic acid II	C₁₆H₁₈O₈	3.64	337.1298	163	Hydrocinnamic acid	Han et al. [27]
23	Kaempferol rutinoside	C₂₇H₃₀O₁₅	3.71	593.2367	285,447	Flavonol	Wen et al. [95]
24	Naringenin hexoside	C₂₁H₂₂O₁₀	3.76	433.1012	271	Flavanone	Ali et al. [4, 5
25	Myricetin galloyl hexoside	C₂₈H₂₃O₁₇	4.35	631.1375	479,316	Flavonol	Abu-Reidah et al. [2]
26	Dihydroxybenzoic acid	C₇H₆O₄	4.40	153	91,83	Hydroxybenzoic acid	Liu et al. [54]
27	(Epi)gallocatechin gallate I	C₂₂H₁₈O₁₁	4.97	457.0955	305,169,125	Flavan-3-ol	Wen et al. [95]
28	Epigallocatechin gallate dihydrate	C₂₂H₂₂O₁₃	5.04	493.0403	457,305,169,125	Flavan-3-ol	Shevchuk et al. [80]
29	Myricetin hexoside	C₂₁H₂₀O₁₃	5.10	479.1303	316,271	Flavonol	Shevchuk et al. [80]
30	Theaflavin	C₂₉H₂₄O₁₂	5.26	563.1412	473,225	Flavan-3-ol	Shevchuk et al. [80]
31	Quercetin-hexosyl-pentosyl-hexoside	C₃₃H₁₀O₂₁	5.43	771.0994	609,463,301	Flavonol	Han et al. [27]
32	Kaempferol rutinoside II	C₂₇H₃₀O₁₅	5.58	593.2497	285	Flavonol	Wen et al. [95]
33	Quercetin-hexosyl-pentosyl-hexoside isomer	C₃₃H₁₀O₂₁	5.62	771.0994	609,463,301	Flavonoid	Han et al. [27]
34	(Epi)gallocatechin gallate II	C₂₂H₁₈O₁₁	5.72	457.0955	305,169,125	Flavan-3-ol	Wen et al. [95]
35	(Epi)catechin-(epi)catechin (Procyanidin B) II	C₃₀H₂₆O₁₂	5.79	577.2106	289	Flavan-3-ol	Wen et al. [95]
36	Quercetin rutinoside	C₂₇H₃₀O₁₆	5.82	609.3044	301,191,107	Flavonol	Kelebek [45]
37	Kaempferol hexosyl deoxyhexosyl hexoside	C₃₃H₄₀O₂₀	5.85	755.2800	593, 447, 285	Flavonol	Kelebek [45]
38	Vitexin	C₂₁H₂₀O₁₀	5.86	431.1213	413,341,311,269	Flavone	He et al. [33]
39	Quercetin hexoside	C₂₁H₂₀O₁₂	5.94	463.1440	301	Flavonol	Wen et al. [95]
40	Kaempferol hexosyl deoxyhexosyl hexoside	C₃₃H₄₀O₂₀	6.02	755.2800	593, 447, 285	Flavonol	Kelebek [45]
41	Epicatechin gallate	C₂₂H₁₈O₁₀	6.17	441.1669	289, 169, 125	Flavan-3-ol	Wen et al. [95]
42	Kaempferol rutinoside III	C₂₇H₃₀O₁₅	6.23	593.1575	285	Flavonol	Wen et al. [95]
43	Quercetin glucuronide	C₂₁H₁₇O₁₃	6.27	477.0204	301	Flavonol	Abu-Reidah et al. [2]
44	(Epi)gallocatechin gallate III	C₂₂H₁₈O₁₁	6.36	457.1225	305,169,125	Flavan-3-ol	Wen et al. [95]
45	Kaempferol-O-hexoside	C₂₁H₂₀O₁₁	6.41	447.1767	283,255,147	Flavonol	Kelebek [45]
46	(Epi)catechin-3-O-(4-O-methyl) gallate	C₂₃H₂₀O₁₀	6.60	455.1686	289,183	Flavan-3-ol	Kelebek [45]
47	(Epi)catechin-hexoside	C₂₁H₂₄O₁₁	6.84	451.0646	289	Flavan-3-ol	Liu et al. [54]
48	Kaempferol-O-trihexosyl-pentoside	C₃₈H₄₇O₂₄	7.19	887.2621	285	Flavonol	Abu-Reidah et al. [1]
49	(Epi)gallocatechin gallate dihydrate II	C₂₂H₂₂O₁₃	8.15	493.0708	457,305,169,125	Flavan-3-ol	Shevchuk et al. [80]
50	Hydroxy-octadecatrienoic acid	C₁₈H₃₀O₃	11.59	293.2600	221,192,71	Fatty acid	Liu et al. [54]
51	Hydroxyoctadecenoic acid	C₁₈H₃₄O₃	14.21	297.115	279,183,155	Fatty acid	Liu et al. [54]
52	Theaflavin-3,3'-digallate	C₄₃H₃₂O₂₀	14.69	867.3915	563,545,527,501,407	Polyphenol	He et al. [33]

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